IN VITRO ANTISICKLING ACTIVITY OF CRUDE AND PARTIALLY PURIFIED FRACTIONS OF AQUEOUS AND METHANOLIC EXTRACTS OF LEAVES, SEEDS AND STEM OF TELFERIA OCCIDENTALIS (FLUTED PUMPKIN)
IN VITRO ANTISICKLING ACTIVITY OF CRUDE AND PARTIALLY PURIFIED FRACTIONS OF AQUEOUS AND METHANOLIC EXTRACTS OF LEAVES, SEEDS AND STEM OF TELFERIA OCCIDENTALIS (FLUTED PUMPKIN)
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Date
2013-05
Authors
ATABO, SAMUEL
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Abstract
This study was carried out to investigate the antisickling activity, membrane stabilizing potential and the effect of extracts on Fe2+/Fe3+ratio in sickled haemoglobin (Hb S). Partial purification, quantitative phytochemical and mineral element analysis of plant extracts were also carried out. The antisickling activity of both aqueous and methanolic extracts of leaf, seeds and stem of Telferia occidentalis at 10.0, 1.0 and 0.1 mg/ml, revealed that the aqueous leaf extracts (10 mg/ml) exhibited highest antisickling activity (64.03%) which was significantly higher (p<0.05) from that of the stem (47.30%) and seed (37.50%). Thin layer chromatography (TLC) of partially purified fractions showed single and double spots of different RF values. They were then pulled together in that respect. Partially purified fractions obtained, recorded improved antisickling effect, with Fraction 3 (a combination of the 31st and 32nd eluents from column chromatography) showing the maximum antisickling activity of 74%. Para-hydroxybenzoic acid and normal saline were used as positive and negative controls respectively. In the membrane stabilizing assay, the methanolic and aqueous stem extracts showed the highest effect of 71.85% and 61.29% respectively. The methanolic leaves extract demonstrated the highest potential decrease in the methemoglobin (mHb) concentration (4.88%) compared with the control that revealed (13.04%). The results of the phytochemicals (quantitative) in plant parts revealed the presence of saponins, alkaloids, flavonoids, cyanogenic glycosides and total phenolics in both extracts of various plant parts. The quantification of mineral composition of plant parts revealed the presence of sodium, potassium, zinc, calcium, magnesium and iron, with a predominant quantity of K, Mg and Fe shown across all extracts. The results provide scientific evidence for the use of Telferia occidentalis in the management of sickled cell disease (SCD) and its possible use in drug development.
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A THESIS SUBMITTED TO THE SCHOOL OF POST GRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENT FOR THE AWARD OF MASTER DEGREE IN BIOCHEMISTRY. DEPARTMENT OF BIOCHEMISTRY, FACULTY OF SCIENCE AHMADU BELLO UNIVERSITY, ZARIA NIGERIA
Keywords
VITRO ANTISICKLING ACTIVITY ,, CRUDE,, PARTIALLY PURIFIED FRACTIONS,, AQUEOUS,, METHANOLIC EXTRACTS,, LEAVES,, SEEDS,, STEM,, TELFERIA OCCIDENTALIS ,, (FLUTED PUMPKIN),