STUDIES ON THE HEPATOPROTECTIVE EFFECTS OF WHITE GRUBS (PHYLLOPHAGA EPHILIDA (SAY)) METHANOLIC EXTRACTS ON LIVER INJURY INDUCED BY CABON TETRACHLORIDEIN RATS
STUDIES ON THE HEPATOPROTECTIVE EFFECTS OF WHITE GRUBS (PHYLLOPHAGA EPHILIDA (SAY)) METHANOLIC EXTRACTS ON LIVER INJURY INDUCED BY CABON TETRACHLORIDEIN RATS
No Thumbnail Available
Date
2015-03
Authors
MARWANA, MUSA
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
This study was carried out to evaluate the hepatoprotective effects of the methanolic extracts of white grubs (anterior and posterior portions) on liver injury induced by carbon tetrachloride (CCl4) in rat (Rattusnorvegicus). The anterior methanolic extract (AME) and posterior methanolic extract (PME) were obtained from the incision of white grub’s abdomen and extracted by using methanol. Male albino rats weighing between 150g- 200g were used. Ten groups of rats (n=6) were administered orally, once in a day for five days with normal saline (negative control), 10mg/kg of silymarin (positive control) or 50mg/kg and 100mg/kg of posterior methanolic extract (PME) and anterior methanolic extract (AME) of white grubs respectively followed by hepatotoxicity induction using CCl4 on day 2 and 3 of the experiment. Blood and liver samples were collected for biochemical and histopathological studies. The extract was also subjected to antioxidant study using 2, 2-diphenyl-1-picrylhadrazyl (DPPH) radical scavenging assay. Rats pretreated with silymarim, PME (50mg/kg) and AME (100mg/kg) and normal control groups showed a significant decrease (p ≤ 0.05) in the levels of aspartate amino transferase (AST), alanine amino transferase (ALT) and alkaline phosphatase (ALP). Histopathologicalstudy showed intense vacuolation, hepatic necrosis and haemorrhage in CCl4 (negative control), PME (100mg/kg), AME (50mg/kg) pretreated groups;while normal hepatic structure was observed in groups pretreated with silymarin, PME (50mg/kg), AME (100mg/kg) and normal control groups. PME exhibited antioxidant activity. The study shows that PME (50mg/kg) and AME (100mg/kg) have hepatopotective effects against CCl4–induced liver damage.
Description
A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARDOF MASTER OF SCIENCE (MSc) DEGREE IN ZOOLOGY DEPARTMENT OF BIOLOGICAL SCIENCES FACULTY OF SCIENCE AHMADU BELLO UNIVERSITY, ZARIA NIGERIA
Keywords
STUDIES,, HEPATOPROTECTIVE EFFECTS,, WHITE GRUBS,, (PHYLLOPHAGA EPHILIDA,, (SAY)),, METHANOLIC EXTRACTS,, LIVER INJURY INDUCED,, CABON TETRACHLORIDEIN RATS,