ISOLATION, CHARACTERIZATION AND ANTIBIOGRAM DETERMINATION OF LISTERIA SPECIES FROM CHICKEN MEAT SOLD IN ZARIA METROPOLIS, KADUNA STATE, NIGERIA

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dc.contributor.authorNUSA, Sadisu Muhammad
dc.date.accessioned2017-09-22T08:05:05Z
dc.date.available2017-09-22T08:05:05Z
dc.date.issued2016-01
dc.descriptionA DISSERTATION SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF A MASTER DEGREE IN MICROBIOLOGY DEPARTMENT OF MICROBIOLOGY, FACULTY OF LIFE SCIENCES AHMADU BELLO UNIVERSITY, ZARIA, NIGERIAen_US
dc.description.abstractThis study was carried out to isolate, characterize and determine the antibiotic susceptibility pattern of Listeria species from chicken meat sold in Zaria metropolis, Kaduna State. A total of 254 samples (154 and 100 raw and processed chicken meat respectively) were collected from three selected districts. Listeria species isolation was performed according to standard procedures using Listeriaprimary and secondary enrichment broth,Oxford and PALCAM agar media. The isolates were identified using biochemical tests and confirmed using Microgen test kit. Antibiotic susceptibility test was carried out on the isolated Listeria species using disk diffusion method. From a total of 154 raw chicken meat samples analysed, 29 (18.8%) were positive for Listeria species. However, no Listeria species was isolated from 100 processed chicken meat samples analysed. No statistical significant differences (P<0.05) was observed in the distribution of Listeria species in raw chicken meat across the 3 locations.Listeria species identified in this study were Listeria ivanovii (44.8%), Listeria grayi (34.5%), Listeria innocua (13.7%), Listeria seeligeri (3.4%) andListeria murrayi (3.4%).However, noListeria monocytogenes was isolated from the samples. Oxford Listeria agar (100%) showed more efficiency in isolation of Listeria species than PALCAM agar (79.3%). The antibiotic susceptibility profile of Listeria species showed thatall the Listeriaspecies isolated were resistant to ampicillin and cefoxitin. A total of 62% of the isolates were resistant to tetracycline and some of the Listeria spp. isolates also showed resistance to Vancomycin (24.7%), chloramphenicol (24.1%), erythromycin (24.1%), gentamicin (34.4%) and clindamycin (34.5%). None of the isolates was resistant to ciprofloxacin.Multiple Antibiotic resistance was also seen in all the Listeria species and multiple antibiotic resistance indices (MARI) was determined to be MARI≥0.2 for all the isolates. The rate at which raw chicken meat sold in Zaria vii metropolis were contaminatedwith Listeria species is an indication that these poultry products could serve as a source of listerial infection for humans.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/9180
dc.language.isoenen_US
dc.subjectANTIBIOGRAM DETERMINATION,en_US
dc.subjectLISTERIA SPECIES,en_US
dc.subjectCHICKEN MEAT,
dc.subjectARIA METROPOLIS,
dc.subjectKADUNA STATE,
dc.subjectNIGERIA
dc.titleISOLATION, CHARACTERIZATION AND ANTIBIOGRAM DETERMINATION OF LISTERIA SPECIES FROM CHICKEN MEAT SOLD IN ZARIA METROPOLIS, KADUNA STATE, NIGERIAen_US
dc.typeThesisen_US
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