ALTERNATE PATHWAYS OF PURINE METABOLISM IN SOME AFRICAN TRYPANOSOMES

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dc.contributor.authorPATRICK, OKAFOR JAMES OGUNUDE
dc.date.accessioned2014-02-10T14:47:31Z
dc.date.available2014-02-10T14:47:31Z
dc.date.issued1983-10
dc.descriptionA thesis submitted to the Postgraduate School, Ahmadu Bello University, in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY Department of : BIOCHEMISTRY Faculty of : SCIENCE Ahmadu Bello University, Zaria. Dates October, 1983en_US
dc.description.abstractThe alternate pathways of purine metabolism in Tradeswoman Congolese; T. vivas and T. Bruce were investigated. Enzymes of purine salvage pathway were detected in the trypanosomes although there were differences in activity. Purine nucleotide hydro lase was found in the gastronomes but purine nucleotide phosphorylate was found only in T. conqol^nse. Adenine microhydrodynamics, an enzyme usually associated with bacteria was detected in T. viva x. The gastronomes studied lack adenine dreaminess but adenine mono-phosphate dreaminess was detected in T. Brucie and T. Congolese. Dreaminess provide points at which interconversion of purines occur; thus differences exist at the level of metabolic interconversion points. A proposal for the salvage of purines in these gastronomes is presented. Transport of purines in T. ambivalence and T. Congolese, measured as uptake of trialed adenine was shown to take place by mediated mechanism. Two transport loci, one transporting adenine and guano-sine, and the other transporting thymine was demonstrated. The inhibitors of nucleotide transport in animal cells, nitrobenzylthioinosine and pyrimidine, did not Inhibit the uptake of adenine by T. ambient. Nitrobenyylthioinosine which also binds tightly to cells did not bind to either T. gambiense or T. congoiense. v. efflux of 3H--thymidine from erythrocytes was used to measure damage to erythrocyte membrane in control mice and mice infected with trypanosomes. The efflux in infected mice was higher than in the controls. Since erythrocytes have a high rate of purine turnover, this may be a major source of exogenous purines for the trypanosomes. The toxicity to mice and rats and trypanocidal activity of the tubercidin was confirmed by the present study. The toxicity of tubercidin was alleviated by intraperitoneal injection of nitrobenzylthioinosinate (5mkj/kg) 5 minutes earlier. Nitrobenzylthioinosinate neither had acute toxic nor trypanocidal effect at the doses tested and did not interfere with the trypanocidal effect of tubercidin. It is suggested that this nucleotide inhibitor. nitrobenzylthioinosinate, should be evaluated in combination with declension analogues in the therapy of African trypanosomiasis.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/989
dc.language.isoenen_US
dc.subjectALTERNATE,en_US
dc.subjectPATHWAYS,en_US
dc.subjectPURINE,en_US
dc.subjectMETABOLISM,en_US
dc.subjectSOME AFRICAN,en_US
dc.subjectTRYPANOSOMESen_US
dc.titleALTERNATE PATHWAYS OF PURINE METABOLISM IN SOME AFRICAN TRYPANOSOMESen_US
dc.typeThesisen_US
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