PREVALENCE AND MOLECULAR STUDIES OF TAENIA SOLIUM AND ASSESSMENT OF SANITARY CONDITIONS OF SOME PIG SLAUGHTER SLABS IN KADUNA METROPOLIS, NIGERIA

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dc.contributor.authorASUKE, Uregwu Agnes Edia
dc.date.accessioned2016-03-17T10:29:05Z
dc.date.available2016-03-17T10:29:05Z
dc.date.issued2015-07
dc.descriptionTHESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFLMENT OF THE REQUIREMENTS FOR THE AWARD OF DOCTOR OF PHILOSOPHY DEGREE IN MICROBIOLOGY DEPARTMENT OF MICROBIOLOGY AHMADU BELLO UNIVERSITY, ZARIA NIGERIAen_US
dc.description.abstractA community-based cross-sectional survey was undertaken to investigate the prevalence and risk factors of Taenia solium infections along with genotyping T. solium and assessment of sanitary conditions of some home-based pig slaughter slabs in Kaduna metropolis, Nigeria. The study was conducted in pig raising areas of Kaduna South and Chikun Local Government Areas in Kaduna metropolis. Three hundred (300) human blood and four hundred and fifty (450) human stool samples were obtained and analysed for cysticercosis and T. solium taeniasis from individuals who gave their consent to participate in the survey. Structured, self-administered questionnaires were used to obtain relevant information on sociodemography and risk factors that might be associated with cysticercosis and taeniasis in the study area. The blood samples were analysed for cysticercal antibodies by use of IgG antibody ELISA, while stool samples were analysed using microscopic examination for taeniid ova, in-house coproantigen ELISA for detection of coproantigens and by molecular assays for confirmation of T. solium present. Taenia solium DNA was extracted from corpoantigen positive stool samples by Qiagen stool mini kit and then amplified by polymerase chain reactions using species-specific primers. Genetic markers targeted were, nuclear internal transcribed spacer regions (ITS I) and mitochondrial cytochrome oxidase C subunit 1 (mt cox 1) genes. The amplified PCR products were partially sequenced, edited and aligned for phylogenetic analysis using BioEdit and CLUSTAL W computer programs. Evolutionary distances were computed by Kimura’s two parameter method, and neighbor joining, bayesian and maximum likelihood trees were constructed using the PAUP vers-5.0 packages. The trees were evaluated using the bootstrap test based on 1000 resamplings. Phylogenetic trees were out group-rooted using nucleotide sequences from Echinococcusen_US
dc.identifier.urihttp://hdl.handle.net/123456789/7479
dc.language.isoenen_US
dc.subjectPREVALENCE,en_US
dc.subjectMOLECULAR STUDIES ,en_US
dc.subjectTAENIA SOLIUM,en_US
dc.subjectASSESSMENT,en_US
dc.subjectSANITARY CONDITIONS,en_US
dc.subjectSOME PIG SLAUGHTER,en_US
dc.subjectSLABS,en_US
dc.subjectKADUNA METROPOLIS,en_US
dc.subjectNIGERIAen_US
dc.titlePREVALENCE AND MOLECULAR STUDIES OF TAENIA SOLIUM AND ASSESSMENT OF SANITARY CONDITIONS OF SOME PIG SLAUGHTER SLABS IN KADUNA METROPOLIS, NIGERIAen_US
dc.typeThesisen_US
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