EVALUATION OF SENSITIVITY OF GENEXPERTS AND CULTURE IN THE DIAGNOSIS OFMYCOBACTERIUM TUBERCULOSISIN PATIENTS ATTENDINGSELECTED HOSPITALS IN ABUJA, NIGERIA.
EVALUATION OF SENSITIVITY OF GENEXPERTS AND CULTURE IN THE DIAGNOSIS OFMYCOBACTERIUM TUBERCULOSISIN PATIENTS ATTENDINGSELECTED HOSPITALS IN ABUJA, NIGERIA.
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Date
2016-07
Authors
DANLADI, Charitymakolo
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Abstract
Tuberculosis is a disease caused by Mycobacterium tuberculosis. A cross sectionalstudy to evaluate the Sensitivityof Genexperts and culture in the diagnosis of M tuberculosis was conducted duringthe period of February,2014 to February, 2015 usingsputum samples among selected out-patients attending Hospitals in FCT Abuja, Nigeria. A total of 671 sputa were collected and analyzed using microscopy, Genexpertsand culture. The occurrence of the isolates using microscopy was 21.9%, (147/671) whileGenexpertsshowed 21.5% (140/671) and culture shows23.4% (157/671) withsignificant difference at P<0.05). Tubercle bacilli identification with Rapid Standard Diagnostic Bioline test was 78.1% (121/671) and Non tuberculous Mycobacterium (NTM) was 21.9% (34/671). There was a high statistically significant difference betweentuberculosis occurrence obtained by microscopy, Genexperts and culture at p<0.05.AlsoStandard diagnostic bioline for Mycobacteriumtuberculosis identification showed a statistically significant difference at P<0.05. The sensitivity of microscopy was 72.1%while that of Genexpertswas 68.8%.While the Specificity were 90.3% and 93.0% respectively. Ofall the four first line drugs used for antibacterial susceptibility testingthe culture isolatesshowed a susceptibility of 92.4% and resistanceof 7.6% to Rifampicin as compared to other drugs while Genexpertsshowed Rifampicin resistance of 0.6% and showed a highly statistical significant difference at p<0.05Cigarette smoking and sex was found to be highly associated to tuberculosis as a risk factor but there was no association in other socio demographic factors consideredFrom this work, culture was found to be still better than microscopy and Genexpertsin diagnosing tuberculosis and detecting the rifampicin resistant tuberculosis than genexperts system.
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A DISSERTATION SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THEAWARD OF AMASTERDEGREE OF SCIENCE IN MICROBIOLOGY DEPARTMENT OF MICROBIOLOGY FACULTY OF SCIENCE AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA
Keywords
EVALUATION,, SENSITIVITY,, GENEXPERTS,, CULTURE,, DIAGNOSIS,, MYCOBACTERIUM,, TUBERCULOSISIN PATIENTS ATTENDINGSELECTED HOSPITALS,, ABUJA,, NIGERIA