EFFECT OF STREPTOCOCCUS THERMOPHILUS ON PERFORMANCE, SURVIVAL AND GUT BACTERIAL FLORA OF CLARIAS GALMAENSISANDCLARIAS GARIEPINUS HYBRID

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Date
2023-06
Authors
IYAKO, Martins Bohotolo
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Abstract
The use of probiotics in aquaculture has shown great potentials in substituting for antibiotics in fish culture as growth promoters and in preventing fish mortality. However, the problem of antimicrobial resistance poses a great threat to human health and has therefore led to the ban of the use of medically important antibiotics in animals as growth promoters. This restriction in the use of antibiotics has led to the discovery and adoption of probiotics. This study was carried out to investigate the probiotic effect of Streptococcus thermophilus isolated from the rumen of cattle on the growth performance, nutrient utilization, survival rate and gut bacterial flora of23DPH hybrid using standard methods. Varying probiotic concentrations 1.5×107CFU/ml, 1.5×108 CFU/mland 1.5×109 CFU/ml of S. thermophilus was administered to three treatment groups ofthe hybrid for 56 days, while the control group was not administered with S. thermophilus. The results showed no significant effect (p≥0.05) of the bacterium on the growth performance and nutrient utilization of the fry but showed significant association (p≤0.05) with its survival rate. The fry survival rate was higher withS.thermophilus concentration of 1.5×109 CFU/ml. The carcass proximate compositions of the fry revealed diminishing protein and lipid contents with increasing S. thermophilus concentrations, while carbohydrate content increased with increasing probiotic concentrations.S. thermophilus did not influence the gut viable bacterial count, however it influenced the gut bacterial isolates and composition. It was observed from this study that S. thermophilus does not have significant effect on the growth performance and nutrient utilization on Clarias hybrid fry but significantly increased its survival rate at 1.5×109 CFU/ml. This study therefore recommend that Streptococcus thermophilus at 1.5×109 CFU/ml can increase surival rate of fry.
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A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF MASTER OF SCIENCE DEGREE IN BIOLOGY DEPARTMENT OF BIOLOGY. FACULTY OF LIFE SCIENCES, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA
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