BACTERIA AND FUNGI ISOLATED FROM SELECTED SACHET WATER MARKETED IN AHMADU BELLO UNIVERSITY, ZARIA MAIN CAMPUS AND THEIR EFFECTS ON DRINKING QUALITY
BACTERIA AND FUNGI ISOLATED FROM SELECTED SACHET WATER MARKETED IN AHMADU BELLO UNIVERSITY, ZARIA MAIN CAMPUS AND THEIR EFFECTS ON DRINKING QUALITY
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Date
2014-12
Authors
THLIZA, Ijai Ayuba
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Abstract
This study focuses on isolating and identifying bacteria and fungi contaminants from sachet waters sold within the University’s main campus, and determines the effects of these microbes on the quality of the sachet water. A sample size of 720 sachet water of 6 different brands were collected at random, and then subjected to storage, while their microbial and physicochemical parameters were analyzed. The colour, taste, odour, turbidity, temperature, pH, electrical conductivity, total dissolved solids, total hardness, chloride, sulphate, nitrate, phosphate.and microbial parameters of the stored samples were analysed weekly, for a period of 6 months. The study utilizedthe modified membrane filter method using commercially prepared Potato Dextrose Agar (PDA) for the isolation of fungi, and the multiple tube fermentation technique with MacConkey broth and Nutrient agar used as reagents for bacteria isolation. Two-way analysis of variance test was employed to analyse the data for fungi and bacteria count at different storage periods. This study revealed that, out of the 720 sachet water samples, fungal species were isolated in 24 and bacterial species were isolated in 54 while 642 were devoid of fungi and bacteria contaminants. Out of the 6 selected brands, fungi were isolated in five brands, while bacteria were isolated in all the six brands. Among the microbes isolatedareAspergillus species,Penicillium species,Fusarium species, Staphylococcus aureus, Escherichia coli and Bacillus cereus were the predominant isolates. All the physicochemical parameters fall within the recommended limits set by Standard Organisation of Nigeria for Drinking Water. The findings revealed the presence of coliform and other pathogens in the samples which indicate risk involved in consumption of such products and therefore could be hazardous to human health.To safeguard the health of the consumers, there is need for regular monitoring of the production and quality by National Agency for Food and Drug Administration and Control.
Description
A THESIS SUBMITTED TO SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD OFMASTER OF SCIENCE (M.Sc.) DEGREE IN BOTANY
Keywords
BACTERIA,, FUNGI, ISOLATED,, SELECTED,, SACHET WATER,, MARKETED,, AHMADU BELLO UNIVERSITY, ZARIA,, MAIN CAMPUS,, EFFECTS,, DRINKING QUALITY.