PREVENTIVE EFFECT OF DIFFERENT LEVELS OF JEW‟S MALLOW (CORCHORUS OLITORIUS LINN) AND SCENT LEAF (OCIMUM GRATISSIMUM LINN) DIET SUPPLEMENTATION ON MNU-INDUCED COLON CANCER IN WISTAR RATS

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dc.contributor.authorOGUNGBEMI, KUNLE
dc.date.accessioned2016-08-04T09:14:30Z
dc.date.available2016-08-04T09:14:30Z
dc.date.issued2015-09
dc.descriptionA THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF A MASTERS DEGREE IN BIOCHEMISTRY DEPARTMENT OF BIOCHEMISTRY FACULTY OF SCIENCE AHMADU BELLO UNIVERSITY, ZARIA NIGERIAen_US
dc.description.abstractThe preventive effect of different levels of Corchorus olitorius Linn and Ocimum gratissimum Linn leaf supplementation on MNU induced colon cancer in wistar rats was investigated using standard method. Weaning rats of 70-90g of weight were fed with vital feed (growers mash) for 2 weeks for acclimatization before being separated into ten (10) groups. All animals except those in the vehicle (normal saline)-treated groups were administered MNU intrarectally thrice a week for 10 weeks and four (4) control groups received normal saline 3 times weekly and for ten (10) weeks. All groups we were given different levels of the supplementation for ten (10) weeks prior to induction and continued on their respective dietary regimen until the termination of the experiment after which the animals were sacrifices for further analysis. Carcinoembryonic antigen assay (CEA) showed that there was a significant increase (P<0.05) with MNU control group (3.47± 0.29) when compared with the normal control (1.17±0.3) and the varying supplemented diet both for Corchorus olitorius Linn and Ocimum gratissimum Linn. Level of CEA also decreases with increasing supplementation. The histopathology of the colon of the MNU control group showed a distorted colonic architecture with distorted blood vessels, hyperchromaticity, and cellular pleomorphism generating anisocytosis and poikilocytosis, with atrophy in the gland, also the section showed necrosis of the lamina propria when compared to the normal control while, normalcy increases with increasing level of dietary supplementation. Thiobarbituric acid reactive substance (TBARS) increased significantly (P<0.05) in both kidney and liver of MNU control group when compared to the normal control group whereas there was significant decrease (p<0.05) in superoxide dismutase (SOD) and catalase (CAT) in MNU control and MNU induced group when compared to the normal control group. Effect of dietary supplementation with Corchorus olitorius Linn and Ocimum gratissimum Linn leaf supplemented diets on haematological parameters showed a significant difference (P<0.05) in the white blood cell (WBC), lymph, hemoglobin (HGB), red viii blood cell (RBC), hematocrit (HCT) , Mean corpuscular haemoglobin concentration (MCHC), Mean corpuscular haemoglobin (MCH) in the MNU control group when compared with that of the normal control group whereas there was no significant difference (P>0.05) between the PLT and MCV of the normal control group and the MNU control group. Therefore, it can be concluded that both Corchorus olitorius Linn and Ocimum gratissimum Linn leave supplementation posses anticancer potentials with the highest activity in the 10% supplementation.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8334
dc.language.isoenen_US
dc.subjectPREVENTIVE EFFECT,en_US
dc.subjectDIFFERENT LEVELS,en_US
dc.subjectJEW‟S MALLOW,en_US
dc.subject(CORCHORUS OLITORIUS LINN),en_US
dc.subjectSCENT LEAF,en_US
dc.subject(OCIMUM GRATISSIMUM LINN),en_US
dc.subjectDIET SUPPLEMENTATION,en_US
dc.subjectMNU-INDUCED COLON CANCER,en_US
dc.subjectWISTAR RATS,en_US
dc.titlePREVENTIVE EFFECT OF DIFFERENT LEVELS OF JEW‟S MALLOW (CORCHORUS OLITORIUS LINN) AND SCENT LEAF (OCIMUM GRATISSIMUM LINN) DIET SUPPLEMENTATION ON MNU-INDUCED COLON CANCER IN WISTAR RATSen_US
dc.typeThesisen_US
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