EVALUATION OF CHLORELLA VULGARIS AS A SOURCE OF FERMENTABLE SUGAR USING AMYLASE PRODUCING BACILLUS CEREUS

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dc.contributor.authorMIGAP, Helen Hoomsuk
dc.date.accessioned2015-03-19T09:58:18Z
dc.date.available2015-03-19T09:58:18Z
dc.date.issued2014-07
dc.descriptionIN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD OF MASTERS DEGREE (M.Sc) IN MICROBIOLOGY DEPARTMENT OF MICROBIOLOGY, FACULTY OF SCIENCE AHMADU BELLO UNIVERSITY, ZARIA NIGERIA JULY, 2014.en_US
dc.description.abstractBacillus cereus was isolated from the soil using mannitol egg York polymixin B agar (MYP) and used for hydrolysis of Chlorella vulgaris starch content. The Dinitrosalicilic acid method was used to determine the amount of reducing sugar produced. Maximum reducing sugar yield of 2.10mg/ml was obtained for pre-treated C. vulgaris biomass, 2.06mg/ml for untreated biomass and 2.56mg/ml for corn starch (control) after 24 hours incubation.When the production parameters where optimized, maximum reducing sugar yield was obtained at a pH of 6.5, temperature of 350C, incubation time of 24 hours and 4% inoculum size for pretreated C. vulgaris biomass. Also, a 1.3 fold reducing sugar yield was enhanced when compared with non-optimized conditions indicating the usefulness of optimization on biochemical processes. The maximum reducing sugar obtained for hydrolysis of Pretreated C.vulgaris biomass is significantly comparable to the reducing sugar yield of corn starch. Also, the carbohydrate content of C. vulgaris was 66% which is comparable to the carbohydrate content of corn (about 73%). Chlorella vulgaris has the potential of being an alternative substrate for fermentable sugar production.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/6275
dc.language.isoenen_US
dc.subjectEVALUATION,en_US
dc.subjectCHLORELLA VULGARIS,en_US
dc.subjectSOURCE,en_US
dc.subjectFERMENTABLE,en_US
dc.subjectSUGAR,en_US
dc.subjectAMYLASE PRODUCING,en_US
dc.subjectBACILLUS CEREUS.en_US
dc.titleEVALUATION OF CHLORELLA VULGARIS AS A SOURCE OF FERMENTABLE SUGAR USING AMYLASE PRODUCING BACILLUS CEREUSen_US
dc.typeThesisen_US
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