EVALUATION OF CO-ADMINISTRATION OF ETHYL ACETATE FRACTION OF Cannabis sativa LEAVES AND ETHANOL ON LEARNING, MEMORY AND THE HISTOMORPHOLOGY OF THE HIPPOCAMPUS IN MICE (Mus musculus)

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dc.contributor.authorILIYA, Bilkisu Talatu
dc.date.accessioned2018-11-22T08:49:38Z
dc.date.available2018-11-22T08:49:38Z
dc.date.issued2017-12
dc.descriptionA THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF MASTER DEGREE IN HUMAN PHYSIOLOGY DEPARTMENT OF HUMAN PHYSIOLOGY, FACULTY OF BASIC MEDICAL SCIENCES, COLLEGE OF HEALTH SCIENCES AHMADU BELLO UNIVERISTY ZARIA NIGERIAen_US
dc.description.abstractSubstance abuse especially amongst young adults constitutes a global problem. Cannabis sativa is a commonly abused illicit drug. Some of its effects include impaired short memory, attention and reduced motor skills. Ethanol is a multiple action depressor of the central nervous system and under its influence, confused and disorganized thinking results. Both drugs have a detrimental effect on prospective memory in adults but users may not be aware of this. This study was therefore aimed at evaluating the co-administration of ethyl acetate fraction of Cannabis sativa leaves and ethanol on Learning, Memory and the histomorphology of the Hippocampus in Mice (Mus Musculus) using the Y-maze and Novel Object Recognition (NOR) paradigms. Fourty eight apparently healthy male albino mice of 20-28 g body weight were assigned into two major groups consisting of four sub-groups each; A, B, C, and D. Each sub group had six mice each (n=6). Sub-groups A, B, C, and D were administered distilled water 1 ml/kg body weight (bwt) (control), 145 mg/kg bwt ethyl acetate fraction of Cannabis sativa leaves, ethanol 20% v/kg bwt and co-administration of 145 mg/kg bwt ethyl acetate fraction of Cannabis sativa leaves and 20% v/kg bwt of alcohol respectively, orally for 21 days. Neurobehavioural assessment was carried out using the Y-maze and Novel Object Recognition (NOR) Paradigms. The Hippocampal histology and volumes were obtained using H & E staining technique and Cavalieri estimator respectively. Data were expressed as meanĀ±SEM. One way ANOVA and Kruskal Wallis tests were used to compare the means at p<0.05. The result of Y-maze test showed a significant increase (P<0.05) in the mean rank of the co-administered group followed by the cannabis group indicating memory impairment when compared to control. NORT result showed a significant decrease (P<0.05) in discrimination and retention indexes in the co-administered group followed by the Cannabis sativa treated group when compared to control. A non-statistical significant (P>0.05) decrease was observed in Hippocampal volume when compared to the control. Histopathological evaluation revealed disorganization and focal necrosis of pyramidal cells of the hippocampus in the co-administered group when compared to the control. It can therefore be inferred from the findings of this study that, co-administration of ethyl acetate fraction of Cannabis sativa leaves with ethanol is more detrimental to hippocampal histoarchitecture with a corresponding memory impairment relative to administration of either.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/10938
dc.language.isoenen_US
dc.subjectEVALUATION,en_US
dc.subjectCO-ADMINISTRATION,en_US
dc.subjectETHYL ACETATE FRACTION,en_US
dc.subjectCannabis sativa LEAVES,en_US
dc.subjectETHANOL,en_US
dc.subjectLEARNING,en_US
dc.subjectMEMORY,en_US
dc.subjectHISTOMORPHOLOGY,en_US
dc.subjectHIPPOCAMPUS,en_US
dc.subjectMICE (Mus musculus)en_US
dc.titleEVALUATION OF CO-ADMINISTRATION OF ETHYL ACETATE FRACTION OF Cannabis sativa LEAVES AND ETHANOL ON LEARNING, MEMORY AND THE HISTOMORPHOLOGY OF THE HIPPOCAMPUS IN MICE (Mus musculus)en_US
dc.typeThesisen_US
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