EFFECTS OF KOLAVIRON ON LYMPHOCYTES PROLIFERATION, EXPRESSION OF TOLL LIKE RECEPTOR-2 AND VASCULAR ENDOTHELIAL GROWTH FACTOR-C GENES IN Wuchereria bancrofti INFECTED BLOOD

Abstract
The anti-proliferative effect and down regulation of vascular endothelial growth factor C and toll like receptor-2 by kolaviron rich portion of Garcinia kola on Wuchereria bancrofti infected blood were investigated. Blood samples were collected from consenting volunteers in Talata mafara, Zamfara State, Nigeria, between the hours of 10pm to 12am, stained with Giemsa and viewed microscopically for presence of microfilariae. Wuchereria bancrofti positive blood samples were cultured in vitro for 72 hours using untreated group as negative control, doxycycline (2μg/ml) treated samples as the positive control while the kolaviron (5μg/ml) treated group was the tested group. Mitotic index, expression of vascular endothelial growth factor-C (VEGF-C), toll like receptor- 2 (TLR-2) were determined using standard procedures, in peripheral blood lymphocytes. Mitotic index was significantly (P<0.05) reduced in the kolaviron treated group compared to negative control. Kolaviron also significantly (P<0.05) downregulated the expression of VEGF-C and TLR-2 when compared with the untreated group. In both cases, the effects of kolaviron was not significantly different (P<0.05) compared to that of doxycycline treated group. Furthermore, strong positive correlations (r) between mitotic index, VEGF-C and TLR-2 expressions were observed. The study concludes that kolaviron rich portion of Garcinia kola exhibited anti-proliferative effect and down regulation of VEGF-C and TLR-2 in Wuchereria bancrofti infected blood. Thus, the results from this study might have unravelled the potential of kolaviron in the management of complications associated with lymphatic filariasis.
Description
A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF MASTER OF SCIENCE DEGREE IN BIOTECHNOLOGY OF BIOCHEMISTRY, FACULTY OF LIFE SCIENCES, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA
Keywords
EFFECT,, KOLAVIRON,, LYMPHOCYTES,, PROLIFERATION,, EXPRESSION,, TOLL LIKE RECEPTOR-2, VASCULAR ENDOTHELIAL,, GROWTH FACTOR-C GENES,, Wuchereria bancrofti,, INFECTED BLOOD,
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