ANTITRYPANOSOMAL POTENTIAL OF THE STEM BARK METHANOLIC EXTRACT OF HYMENOCARDIA ACIDA AGAINST TRYPANOSOMA EVANSI

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dc.contributor.authorNWOSU, OCHEZE NNEOMA
dc.date.accessioned2015-01-27T07:58:24Z
dc.date.available2015-01-27T07:58:24Z
dc.date.issued2013-03
dc.descriptionA RESEARCH THESIS SUBMITTED TO THE POSTGRADUATE SCHOOL AHMADU BELLO UNIVERSITY, ZARIA NIGERIA IN PARTIAL FULFILMENT FOR THE AWARD OF MASTERS OF SCIENCE DEGREE IN BIOCHEMISTRY DEPARTMENT OF BIOCHEMISTRY, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA. MARCH, 2013en_US
dc.description.abstractAntitrypanosomal activities of methanolic extract of stem bark of Hymenocardia acida was evaluated in vitro and in vivo in Trypanosome evansi infected rats. In vitro study was carried out using three extracts (Methanol, petroleum ether, chloroform). Methanolic extract which was the most active extract was used for the in vivo study. LD50 of the methanolic extract was calculated to be 245mg/kg. In vivo studies revealed that infected rats treated with methanolic extract of Hymenocardia acida at 90mg/kg/body weight showed significant decrease in level of parasitaemia thereby extending the survival days of the rats compared to control. In the prophylactic studies, groups given 2 and 3 doses of the methanolic extract before infection did not express parasitaemia even after 30 days post infection. Results of the phytochemical analysis showed the methanolic extract contains flavonoids, triterpines, steroids, saponins, cardiac glycosides, glycosides, tannins and alkaloids. Chromatographic studies carried out on the methanolic extract revealed 2 fractions (Fractions 1and Fraction 2). In vitro and in vivo study of the 2 fractions revealed that fraction 2 was the most active although both fractions were able to extend the survival days of the rats compared to control. GCMS of the 2 fractions indicated presence of n-Hexadecanoic acid and Octadecanoic acid, 1-Eicosene and 17-Pentatriacontene which can be classified as simple aliphatic compounds has been implicated in the result as the active principle in the treatment of trypanosomosis.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/5871
dc.language.isoenen_US
dc.subjectANTITRYPANOSOMAL,en_US
dc.subjectPOTENTIAL,en_US
dc.subjectSTEM,en_US
dc.subjectMETHANOLIC,en_US
dc.subjectHYMENOCARDIA,en_US
dc.subjectACIDA,en_US
dc.subjectTRYPANOSOMA,en_US
dc.subjectEVANSIen_US
dc.titleANTITRYPANOSOMAL POTENTIAL OF THE STEM BARK METHANOLIC EXTRACT OF HYMENOCARDIA ACIDA AGAINST TRYPANOSOMA EVANSIen_US
dc.typeThesisen_US
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