ANTIMICROBIAL AND WOUND HEALING POTENTIALS OF LEAF AND STEM EXTRACTS OF CATHARANTHUS ROSEUS, MITRACARPUS SCABER AND SENNA ALATA IN RATS
ANTIMICROBIAL AND WOUND HEALING POTENTIALS OF LEAF AND STEM EXTRACTS OF CATHARANTHUS ROSEUS, MITRACARPUS SCABER AND SENNA ALATA IN RATS
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Date
2011-03
Authors
MALLUM, MIDAWA SABASTIAN
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Abstract
The leaves and stems of Catharanthus roseus (L), Mitracarpus scaber (L)
and Senna alata (L) were screened for their antimicrobial and
phytochemical properties. Ethanol was used as the solvent of extraction
of the plant samples. The leaves and stems extracts of C. roseus, M.
scaber and S. alata were investigated at 250 and 500mg for wound
healing activities on excision model in laboratory Rats. The
antimicrobial activities of the plant samples were tested against
standard organisms, Staphylococcus aureus (ATCC 02101), Escherichia
coli (ATTC 11775), Pseudosomonas aeruginosa (ATTC 10145) and the
laboratory isolates of Candida albicans and Aspergillus niger. The plant
samples were also screened for the bioactive constituents which
includes Carbohydrates, Glycosides, Free anthraquinones, Combined
anthracene, Cardiac glycosides, Steroids, Triterpenes, Flavonoids,
Tannins, Alkaloids and Saponins. The Free anthraquinones were absent
in all the plant samples tested. Glycosides were absent in the leaf and
the Combined anthracene was absent in the stem extracts of
Mitracarpus scaber. The stem extracts of Senna alata also revealed the
absence of Flavonoids, Tannins and Alkaloids while the other
phytoconstituents remain the same in all the plant samples. The
activities of the extracts showed both bacteriostatic and fungistatic
effects. The MICs and the MBC/MFC, ranges from 0.0625 – 0.5mg. The
ethanolic extracts of all the plant samples did not exert toxic effects on
the Mice used in the toxicity screening. The animals treated with the
ethanolic extracts of the plants used in this study showed a significant
wound healing response compared to those that received negative
(placebo) treatment. The mean wound healing response of the extracts
treated groups range from 16.01 – 20.88 days. While the positive was
14.60 days and the negative was 22.91 days. The wound healing
properties of the plants may be associated with their antimicrobial
activity and also the observed photochemical. The percentage wound
healing contractions along with the antimicrobial activities support the
use of the three plant samples in the topical management of wound
healing. Thus the traditional claims of the uses of the plants in the
treatment of wounds are therefore justified.
Description
A THESIS SUBMITTED TO THE POSTGRADUATE
SCHOOL, AHMADU BELLO UNIVERSITY, ZARIA, IN
PARTIAL FULFILLMENT OF THE REQUIREMENT
FOR AWARD OF MASTER SCIENCE EDUCATIONAL
BIOLOGY, DEPARTMENT OF BIOLOGICAL
SCIENCES, AHMADU BELLO UNIVERSITY, ZARIA
NIGERIA.
30th March, 2011
Keywords
ANTIMICROBIAL,, WOUND HEALING, POTENTIALS,, LEAF AND STEM,, EXTRACTS,, CATHARANTHUS,, ROSEUS,, MITRACARPUS,, SCABER,, SENNA,, ALATA,, RATS