ISOLATION, CHARACTERIZATION AND ANTIBIOTIC SUSCEPTIBILITY PATTERN OF ESCHERICHIA COLI PATHOTYPES AMONG CHILDREN WITH DIARRHOEA IN ZARIA, NIGERIA
ISOLATION, CHARACTERIZATION AND ANTIBIOTIC SUSCEPTIBILITY PATTERN OF ESCHERICHIA COLI PATHOTYPES AMONG CHILDREN WITH DIARRHOEA IN ZARIA, NIGERIA
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Date
2014-10
Authors
SANI, Auwal
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Abstract
Diarrhoea is one of the foremost public health problems worldwide especially among Children
under five years in developing countries. Only few studies have investigated the epidemiology
and virulence of Escherichia coli pathotypes in South-Eastern and South-Western Nigeria
leaving the Northern part of the country unstudied. The isolation and biochemical identification
of the isolates were performed using standard microbiological procedures. Antimicrobial
Susceptibility testing was performed by disc diffusion method using modified Kirby-Bauer
sensitivity testing techniques. Multiplex PCR test was performed to characterize the E. coli
isolates into their respective pathotypes. A total of 100 isolates of Escherichia coli were
obtained from the diarrhoeic stool of 222 paediatric patients under the age of five (5) years. The
result of antimicrobial susceptibility profile of the isolates showed that, 86% of the E. coli
isolates were resistant to Amoxicillin, 85% to Cotrimoxazole, 81% to Erythromycin, 40% to
Chloramphenicol, 24% to Augmentin (Amoxicillin/Clavulanic acid), 23% to Ciprofloxacin, 23%
to Ceftriaxone, and 22% to Gentamicin. The multiple antibiotic resistance index of 0.1 was
obtained in 11% of the E. coli isolates, 0.2 in 10%, 0.3 in 19%, 0.5 in 24%, 0.6 in 15%, 0.7 in
11%, 0.8 in 6%, and 1.0 in 2%. Multiple antibiotic resistance (MAR) index of the E. coli isolates
in this study showed that 58% have MAR index of ≥ 0.5. Following β-lactamase production test,
55.8% of the Amoxicillin resistant isolates were found to be β-lactamase positive while 44.2%
were found to be β-lactamase negative. From the results obtained, only one (1) percent of the
isolates was found to harbor the virulence gene out of the 100 E. coli isolated from the diarrhoea
stools of children employed in this study. This virulence gene was found to be
dispersin/antiaggregation protein (aap), a characteristic of Enteroaggregative E. coli ( EAEC).
Description
A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES,
AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA.
IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD
OF A MASTER DEGREE (M.Sc) IN PHARMACEUTICAL MICROBIOLOGY
DEPARTMENT OF PHARMACEUTICS AND PHARMACEUTICAL
MICROBIOLOGY,
FACULTY OF PHARMACEUTICAL SCIENCES
AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA
OCTOBER, 2014
Keywords
ISOLATION,, CHARACTERIZATION,, ANTIBIOTIC,, SUSCEPTIBILITY,, PATTERN,, ESCHERICHIA,, COLI PATHOTYPES,, CHILDREN,, DIARRHOEA,, ZARIA, NIGERIA