ISOLATION, CHARACTERIZATION AND DETERMINATION OF THE LYTIC PROPERTY OF BACTERIOPHAGES ON SALMONELLA SPECIES

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dc.contributor.authorBALA, DONNA TIGE
dc.date.accessioned2017-07-24T15:24:33Z
dc.date.available2017-07-24T15:24:33Z
dc.date.issued2016-11
dc.descriptionA DISSERTATION SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF A MASTER DEGREE IN MICROBIOLOGY DEPARTMENT OF MICROBIOLOGY, FACULTY OF LIFE SCIENCES, AHMADU BELLO UNIVERSITY ZARIA, NIGERIAen_US
dc.description.abstractThe control of pathogens such as Salmonella is vital to control economic loss, animal and human morbidity and mortality. The use of antibiotics in the control of these pathogens is facing challenges such as development of resistance and adverse side effects. There is need for other antibacterial agents to be used together with or as alternative to antibiotics. Bacteriophages which infect specific bacteria show specialty in this aspect and is known to be a potential bio-control agent against pathogenic bacteria like Salmonella. This study was aimed at isolating, characterizing and determining the lytic property of bacteriophages on Salmonella species obtained in the course of the study from an egg and clinical isolate from the University‟s Sickbay. Two Salmonella species were used for the study -one was a clinical isolate obtained from the University‟s health services which was isolated from human source and the other was isolated from inside an egg. Seven Bacteriophages {three for each and the reference phage (Salmonelex which was purchased from Microes Food Safety, Netherlands) for both} that are lytic against the two Salmonella species were isolated (from the central collection point of the university‟s drainage system, the dam at Kwanan kurmi and soil from the farmlands and open fields where animals usually graze around the dam), cultivated and concentrated. Their antibacterial effects on the two Salmonella isolates were compared in vitro with that of two antibiotics (Ciprofloxacin and Chloramphenicol) and that of their combination with each antibiotic (1DWC, 1DWCip, 1KSC, 1KSCip, 1KWC, 1KWCip, 1RC, 1RCip, 2DWC, 2DWCip, 2KSC, 2KSCip, 2KWC, 2KWCip, 2RC and 2RCip). The phages were found to be as effective as the antibiotics (like in the comparison between 1R and Chloramphenicol, 2DW and Ciprofloxacin, 2KS and Ciprofloxacin and between 2R and Chloramphenicol); in some cases they were more effective than the antibiotics (such as the comparison between ix 1DW and Chloramphenicol or Ciprofloxacin, 1R and Chloramphenicol or Ciprofloxacin and between 2R and Ciprofloxacin) and in some cases (such as comparison between Chloramphenicol and 1KS, 1KW, 2DW, 2KS or 2KW and between Ciprofloxacin and 1KW or 1KS) they were less effective. In some cases the combination of the phages and the antibiotics (such as the combination of Chloramphenicol with 1KS, 1KW, 1R or 2KW and Ciprofloxacin with 1KS, 1KW, 2KS or 2KW) gave synergistic effect. It was evident these phages and their combinations with antibiotics can be potential control agents of Salmonella species. Bacteriophages can be used as bio-control agents against Salmonella and they can equally be used as complement or alternative to antibiotics such as Chloramphenicol and Ciprofloxacin in the control of Salmonella infectionen_US
dc.identifier.urihttp://hdl.handle.net/123456789/9103
dc.language.isoenen_US
dc.subjectLYTIC PROPERTY OF BACTERIOPHAGES,en_US
dc.subjectISOLATION,en_US
dc.subjectSALMONELLA SPECIES,en_US
dc.titleISOLATION, CHARACTERIZATION AND DETERMINATION OF THE LYTIC PROPERTY OF BACTERIOPHAGES ON SALMONELLA SPECIESen_US
dc.typeThesisen_US
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