INFLUENCE OF ZINC SUPPLEMENTATION ON THE PATHOLOGY OF SUBCHRONIC EXPOSURE OF WISTAR RATS TO GLYPHOSATE

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dc.contributor.authorTIZHE, Emmanuel Vandi
dc.date.accessioned2014-02-10T11:34:07Z
dc.date.available2014-02-10T11:34:07Z
dc.date.issued2012-09
dc.descriptionA THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILMENT FOR THE AWARD OF THE DEGREE OF MASTER OF SCIENCE IN VETERINARY PATHOLOGY DEPARTMENT OF PATHOLOGY, FACULTY OF VETERINARY MEDICINE, AHMADU BELLO UNIVERSITY, ZARIAen_US
dc.description.abstractGlyphosate used in this study is the active ingredient of the glyphosate – based herbicide, Bushfire®. The study was conducted to determine the influence of zinc supplementation on the pathology of subchronic exposure of Wistar rats to glyphosate. Sixty adult male and female Wistar rats were used. Twelve rats were used to determine the LD50 which was 3750 mg kg-1 and forty eight were randomized in to six groups of eight rats each, four males and four females. Group I (DW) served as control and were administered only distilled water (2 ml kg-1), group II (Z) were administered zinc only (50 mg kg-1), group III (G) were administered glyphosate only (375 mg kg-1, 10 % of the LD50), group IV (Z + G) were pretreated with zinc (50 mg kg-1) and glyphosate was administered (375 mg kg-1) while those in group V (GC) were administered glyphosate (14.4 mg kg-1, 1: 50 glyphosate : distilled water respectively, as being used for field application) and those in group VI (Z + GC) were pretreated with zinc (50 mg kg-1) and glyphosate was administered (14.4 mg kg-1 , 1 : 50 glyphosate : distilled water respectively). The treatment regimens were administered orally by gavage once daily for eight weeks. At the end of the study period, blood samples were collected and analysed for haematological and serum – biochemical parameters which included packed cell volume, hemoglobin, total leucocyte count, absolute and differential leucocyte count, total protein, albumin, globulin, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, Na+, K+, Cl-, HCO- 3, Ca2+, PO- 4, urea and creatinine. Alterations in the values of haematological and serum – biochemical parameters induced by glyphosate exposure were differentially ameliorated by pretreatment with zinc. Histopathologic examination revealed neuronal degeneration, degenerated hepatic cells especially at the portal areas and congested central vein,depopulated splenic cells in both red and white pulps, degenerated glandular pancreatic acinar cells, glomerular degeneration, few mononuclear cells infiltration into the interstices with renal tubular necrosis and degenerated mucosal epithelial cells of the stomach that involved the muscularis mucosa and the glands. No observable lesions in the testis and the uterus. Supplementation with zinc ameliorated the microscopic lesions induced by glyphosate exposure in these organs. In conclusion, this study showed the ameliorative effect of zinc on subchronic glyphosate – induced pathological changes in Wistar rats. Keywords: Glyphosate, Zinc, Haematology, Serum – biochemistry, Pathology and Ameliorate.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/934
dc.language.isoenen_US
dc.subjectINFLUENCEen_US
dc.subjectZINCen_US
dc.subjectSUPPLEMENTATIONen_US
dc.subjectPATHOLOGYen_US
dc.subjectSUBCHRONICen_US
dc.subjectEXPOSUREen_US
dc.subjectWISTARen_US
dc.subjectRATSen_US
dc.subjectGLYPHOSATEen_US
dc.titleINFLUENCE OF ZINC SUPPLEMENTATION ON THE PATHOLOGY OF SUBCHRONIC EXPOSURE OF WISTAR RATS TO GLYPHOSATEen_US
dc.typeThesisen_US
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