INFLUENCE OF ZINC SUPPLEMENTATION ON THE PATHOLOGY OF SUBCHRONIC EXPOSURE OF WISTAR RATS TO GLYPHOSATE
INFLUENCE OF ZINC SUPPLEMENTATION ON THE PATHOLOGY OF SUBCHRONIC EXPOSURE OF WISTAR RATS TO GLYPHOSATE
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Date
2012-09
Authors
TIZHE, Emmanuel Vandi
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Abstract
Glyphosate used in this study is the active ingredient of the glyphosate – based herbicide,
Bushfire®. The study was conducted to determine the influence of zinc supplementation on
the pathology of subchronic exposure of Wistar rats to glyphosate. Sixty adult male and
female Wistar rats were used. Twelve rats were used to determine the LD50 which was 3750
mg kg-1 and forty eight were randomized in to six groups of eight rats each, four males and
four females. Group I (DW) served as control and were administered only distilled water (2
ml kg-1), group II (Z) were administered zinc only (50 mg kg-1), group III (G) were
administered glyphosate only (375 mg kg-1, 10 % of the LD50), group IV (Z + G) were
pretreated with zinc (50 mg kg-1) and glyphosate was administered (375 mg kg-1) while
those in group V (GC) were administered glyphosate (14.4 mg kg-1, 1: 50 glyphosate :
distilled water respectively, as being used for field application) and those in group VI (Z +
GC) were pretreated with zinc (50 mg kg-1) and glyphosate was administered (14.4 mg kg-1
, 1 : 50 glyphosate : distilled water respectively). The treatment regimens were
administered orally by gavage once daily for eight weeks. At the end of the study period,
blood samples were collected and analysed for haematological and serum – biochemical
parameters which included packed cell volume, hemoglobin, total leucocyte count, absolute
and differential leucocyte count, total protein, albumin, globulin, aspartate
aminotransferase, alanine aminotransferase, alkaline phosphatase, Na+, K+, Cl-, HCO-
3,
Ca2+, PO-
4, urea and creatinine. Alterations in the values of haematological and serum –
biochemical parameters induced by glyphosate exposure were differentially ameliorated by
pretreatment with zinc. Histopathologic examination revealed neuronal degeneration,
degenerated hepatic cells especially at the portal areas and congested central vein,depopulated splenic cells in both red and white pulps, degenerated glandular pancreatic
acinar cells, glomerular degeneration, few mononuclear cells infiltration into the interstices
with renal tubular necrosis and degenerated mucosal epithelial cells of the stomach that
involved the muscularis mucosa and the glands. No observable lesions in the testis and the
uterus. Supplementation with zinc ameliorated the microscopic lesions induced by
glyphosate exposure in these organs. In conclusion, this study showed the ameliorative
effect of zinc on subchronic glyphosate – induced pathological changes in Wistar rats.
Keywords: Glyphosate, Zinc, Haematology, Serum – biochemistry, Pathology and
Ameliorate.
Description
A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES,
AHMADU BELLO UNIVERSITY, ZARIA
IN PARTIAL FULFILMENT FOR THE AWARD OF THE DEGREE OF MASTER OF
SCIENCE IN VETERINARY PATHOLOGY
DEPARTMENT OF PATHOLOGY, FACULTY OF VETERINARY MEDICINE,
AHMADU BELLO UNIVERSITY, ZARIA
Keywords
INFLUENCE, ZINC, SUPPLEMENTATION, PATHOLOGY, SUBCHRONIC, EXPOSURE, WISTAR, RATS, GLYPHOSATE