EFFECT OF ASPERGILLUS NIGER AMYLASES ON CASSAVA STARCH HYDROLYSIS

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dc.contributor.authorNELSON, AKEME-ESUOMINI OSARIBIE
dc.date.accessioned2014-02-25T14:25:54Z
dc.date.available2014-02-25T14:25:54Z
dc.date.issued2013-02
dc.descriptionA THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD OF A MASTER DEGREE IN CHEMICAL ENGINEERING. DEPARTMENT OF CHEMICAL ENGINEERING, FACULTY OF ENGINEERING AHMADU BELLO UNIVERSITY, ZARIA NIGERIA FEBRUARY, 2013en_US
dc.description.abstractThe enzymatic hydrolysis of cassava starch to glucose has been studied. Aspergillus niger was isolated from deteriorated groundnut seeds and used as the amylase producer source. The effect of various process variables such as culture age, mycelia mass loading, pH, temperature and substrate (cassava starch) concentration were investigated in order to optimize the hydrolysis process. The A. niger amylases exhibited optimum activity at pH and temperature of 4.8 and 60oC respectively. The cells appear most viable on the fifth day. Results obtained from this study indicated that increase in cassava starch concentration from 0.3 to 1.5 g/l was accompanied with a proportionate increase in glucose concentration from 0.239 to 0.310 g/l and decrease in glucose yield, falling from 74% to 18%. In addition, increase in mycelia mass loading resulted to increase in both the concentration and yield of glucose. An initial rate method was employed in estimating the kinetic parameters using Michealis-Menten model. The Langmuir form of the Michealis-Menten model gave a high regression coefficient (0.9939) and the values of the maximum reaction velocity (rmax) and Michaelis constant (Km) were found to be 2.42*10-3 g/l.min and 0.221 g/l respectivelyen_US
dc.identifier.urihttp://hdl.handle.net/123456789/2672
dc.language.isoenen_US
dc.subjectEFFECT,en_US
dc.subjectASPERGILLUS NIGER AMYLASES,en_US
dc.subjectCASSAVA STARCH,en_US
dc.subjectHYDROLYSIS.en_US
dc.titleEFFECT OF ASPERGILLUS NIGER AMYLASES ON CASSAVA STARCH HYDROLYSISen_US
dc.typeThesisen_US
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