STUDIES ON ANTIANAEMIC POTENTIAL OF METHANOL EXTRACT OF RED CREOLE ONIONS (Allium cepa) IN PHENYLHYDRAZINE-INDUCED HAEMOLYTIC ANAEMIA IN RATS
STUDIES ON ANTIANAEMIC POTENTIAL OF METHANOL EXTRACT OF RED CREOLE ONIONS (Allium cepa) IN PHENYLHYDRAZINE-INDUCED HAEMOLYTIC ANAEMIA IN RATS
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Date
2018-01
Authors
ZAKARI, AMINA
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Abstract
The antianemic potential of crude methanol extract of red creole onion cultivar was investigated in phenylhydrazine-induced anaemic albino rats. LD50 of the crude methanol extract was found to be greater than 5000mg/kg/b.w. Thirty five (35) apparently healthy albino rats were grouped into seven groups of five (5) rats per group for the determination of the antianemic effect of the crude methanol extract, and groups two (II) to six (VI) were intraperitoneally administered with phenylhydrazine (10mg/kg/b.w/day) for 4 days. The study was carried out for a period of 21 days in which there was subsequent treatment of groups four (1V) to six (V1) with crude methanol extract of red creole onions at three different doses of 200,400 and 600mg/kg/b.w/day respectively, while Bioferon (0.23ml/kg/b.w) was used as a standard drug for positive control group. Haematological parametres were determined on weekly basis. Non-significant(p>0.05) increase in Hb, PCV and RBC concentrations were observed for all the treated groups at the end of the experiment, however, the RBC of the group that receives the highest dose (600mg/kg/b.w) decreased slightly after two weeks treatment with the extract. Non-significant(p>0.05) decrease in reticulocytes counts were also observed for all the treated anemic groups. The non-significant increase in all the parameters were not comparable with the standard drug (Bioferon) which shows a significant (p<0.05)increase in Hb, PCV and RBC as well as decrease in reticulocytes counts after three weeks treatment with the drug. The phytochemicals found in red creole onion extract include alkaloids, flavonoids, cardiac glycosides and phenolics. Terpenoids, Saponins and cyanogenic glycosides were absent. These results suggest that red creole onion does not possess antianaemic effect, especially as compared with the standard drug.
Description
A THESIS SUBMITTED TO POSTGRADUATE SCHOOL AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE
AWARD OF MASTER DEGREE (M.Sc) IN BIOCHEMISTRY
DEPARTMENT OF BIOCHEMISTRY
FACULTY OF LIFE SCIENCES
AHMADU BELLO UNIVERSITY,
ZARIA-NIGERIA
Keywords
STUDIES,, ANTIANAEMIC POTENTIAL,, METHANOL EXTRACT,, RED CREOLE ONIONS,, PHENYLHYDRAZINE-INDUCED HAEMOLYTIC ANAEMIA,, RATS