PHYTOCHEMICAL AND INHIBITION STUDIES OF GARCINIA KOLA HECKEL (GUTTIFERAE) SEED EXTRACTS ON SOME KEY ENZYMES INVOLVED WITH DIABETES

Simple item page
dc.contributor.authorZAKARIYA, Ali Muhammad
dc.date.accessioned2017-01-24T07:45:15Z
dc.date.available2017-01-24T07:45:15Z
dc.date.issued2015-11
dc.descriptionA DISSERTATION SUBMITTED TO THE SCHOOL OF POST GRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD OF A MASTER`S DEGREE IN PHARMACOGNOSY DEPARTMENT OF PHARMACOGNOSY AND DRUG DEVELOPMENT FACULTY OF PHARMACEUTICAL SCIENCES, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIAen_US
dc.description.abstractGarcinia kola is an Angiosperm belonging to the family Guttiferae. It is known in commerce as bitter kola. The plant seeds have been used in the treatment of a wide range of diseases including diabetes, and its importance in folkloric medicine as a purgative, mastcatory, aphrodisiac etc. is eminent. Diabetes mellitus is a metabolic disorder of multiple etiologies characterized by chronic hyperglycemia leading to severe complications such as neuropathy, nephropathy, retinopathy and foot ulcer. n-Hexane, ethyl acetate and methanol extracts were prepared successively in a soxhlet apparatus at 50ºC. Qualitative phytochemical screening was carried out. Column chromatographic analysis was carried out on the ethyl acetate extract and the structure of the isolated compound was elucidated via Gas Chromatography-Mass Spectrophotometry and Fourier Transformed-Infra Red spectroscopy. Pancreatic α-amylase and intestinal α-glucosidase were extracted from porcine pancrease and rat small intestine under specified conditions. Steroids/triterpenes, phenolics, flavonoids, cardiac glycosides, alkaloids, coumarins and phlobatannins were detected. Methanol, ethyl acetate and n-hexane extracts inhibited α-amylase with IC50 = 0.78 ± 0.32 mg/ml, 3.44 ± 3.46 mg/ml, 4.89 ± 4.62 and α-glucosidase IC50 = 2.67 ± 0.74 mg/ml, 1.68 ± 1.27 mg/ml, 10.29 ± 4.08 mg/ml respectively. The compound ZAAK was isolated from ethyl acetate extract. Fourier Transformed-Infrared spectra revealed the presence of carboxylic acid and an ester in ZAAK. Total ion chromatogram of ZAAK revealed three major peaks corresponding to ZAAK1 ZAAK2 and ZAAK3. The mass spectra identified ZAAK1 ZAAK2 and ZAAK3 as 1 pentadecanecarboxylic acid, (Z)-11-Octadecenoic acid and octadecanoic acid, 2-(2hydroxyethoxy) ethyl ester respectivelyen_US
dc.identifier.urihttp://hdl.handle.net/123456789/8475
dc.language.isoenen_US
dc.subjectPHYTOCHEMICAL,en_US
dc.subjectINHIBITION STUDIES,en_US
dc.subjectGARCINIA KOLA HECKEL,en_US
dc.subject(GUTTIFERAE),en_US
dc.subjectSEED EXTRACTS,en_US
dc.subjectKEY ENZYMES,en_US
dc.subjectDIABETES,en_US
dc.titlePHYTOCHEMICAL AND INHIBITION STUDIES OF GARCINIA KOLA HECKEL (GUTTIFERAE) SEED EXTRACTS ON SOME KEY ENZYMES INVOLVED WITH DIABETESen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
PHYTOCHEMICAL AND INHIBITION STUDIES OF GARCINIA KOLA HECKEL (GUTTIFERAE) SEED EXTRACTS ON SOME KEY ENZYMES INVOLVED WITH DIABETES.pdf
Size:
1.55 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.62 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
PHARMACEUTICAL SCIENCES