COMPARATIVE STUDY OF GLUTAMIC ACID PRODUCTION BY WILD-TYPE AND MUTANT STRAINS OF CORYNEBACTERIUM GLUTAMICUM
COMPARATIVE STUDY OF GLUTAMIC ACID PRODUCTION BY WILD-TYPE AND MUTANT STRAINS OF CORYNEBACTERIUM GLUTAMICUM
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Date
2016-10
Authors
BISHIR, Musa
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Abstract
Several different lignocellulosic biomass of agricultural origin hold remarkable potential for conversion into commodity products presenting dual advantage of sustainable resource supply and environmental quality. There is generally an increasing demand for amino acids especially L-glutamic acid as growth promoting factor, as well as flavour enhancer in foods. The present study was an investigation on comparative L-glutamic acid production by wild-type and a mutant strain of Corynebacterium glutamicum (CGNTA) using rice husk pretreated with 1.0M H2SO4 and 1.0M KOH. The acid-treated and alkali-treated rice husk with high carbohydrate content of 64.25% and 76.37% respectively as determined, were used for the production of glutamic acid by submerged fermentation. The acid-treated and alkali-treated rice husk at concentration of 4% gave the highest glutamic acid yield of 27.84g/L and 15.72g/L respectively with the developed mutant strain (CGNTA) under predetermined optimum fermentation conditions (30oC, pH 7.0, 4% substrate concentration and 7% inoculum size). In contrast, lower yields of 10.40g/L and 9.08g/L respectively were obtained with the wild type strain under similar optimum culture conditions. Out of four parameters optimized, all were found to significantly (p˂0.05) influence glutamate production from both the acid and alkali-treated rice husk by the CGNTA. Similarly, all parameters except variation in the concentrations of the acid and alkali-treated rice husk (p˂0.05) were found to be significant on the performance of the wild-type strain in glutamate production. Acid-treated rice husk hydrolysate was determined to be a better substrate for L-glutamate production by the CGNTA mutant than the wild type strain of C. glutamicum. The mutant strain (CGNTA) developed could, therefore, be useful in the industrial production of glutamic acid using rice husk as substrate pretreated with acid. This may perhaps form the basis of starting a microbial L-glutamate production industry from rice husk as substrate in this locality and Nigeria as a whole
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A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA
IN PARTIAL FULFILLMENT OF THE REQUREMENTS FOR THE
AWARD OF MASTER DEGREE IN MICROBIOLOGY DEPARTMENT OF MICROBIOLOGY, FACULTY OF SCIENCE, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA
Keywords
COMPARATIVE,, STUDY,, GLUTAMIC,, PRODUCTION,, WILD-TYPE,, MUTANT,, STRAINS,, CORYNEBACTERIUM,, GLUTAMICUM,