ISOLATION AND CHARACTERIZATION OF ANTIBACTERIAL COMPOUNDS FROM AERIAL PARTS OF CLAVILLIA (MIRABILIS JALAPA LINN)
ISOLATION AND CHARACTERIZATION OF ANTIBACTERIAL COMPOUNDS FROM AERIAL PARTS OF CLAVILLIA (MIRABILIS JALAPA LINN)
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Date
2017-08
Authors
SADIQ, MAS’UD ENEJI,
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Abstract
This study examined the antibacterial effects of some compounds present in the medicinal plant Mirabilis jalapa. Extracts of the aerial parts were obtained by soxhlet extraction using organic solvents in increasing order of polarity. Each extract was subsequently screened for bactericidal activity against clinical isolates of selected entero-pathogenic bacteria. Active fractions were subjected to several steps of chromatography techniques resulting in isolation of four compounds. The structure of each compound was elucidated using data obtained from 1D and 2D Nuclear Magnetic Resonance (NMR), Fourier Transform Infra Red (FTIR) and Ultraviolet (UV) spectroscopic techniques. Molecular masses of the compounds were also determined by
Electron Impact ionization-Mass Spectrometry (EI-MS) and Electrospray Ionization-Mass Spectrometry (ESI-MS). Administration, Distribution, Metabolism and Toxic (ADMETox) properties of each isolated compound was evaluated by in silico methods while in-vitro antimicrobial assay was conducted on compounds predicted to possess drug-like properties suitable for oral route administration. The methanol extract showed Minimum Inhibitory Concentration (MIC) values of 2.5 mg/ml against Bacillus cereus, Eschericia coli, Salmonella typhi and Shigella dysentariea while 10 mg/ml was the Minimum Bactericidal Concentration (MBC) required to completely kill the organisms. Trolline (compound 1), isorhamnetin (compound 2), and tryptophan (compound 3) were isolated from methanol extract while β-sitosterol (compound 4) was isolated from dichloromethane extract. Compound 1 was selected for bioassay based on cumulated results on in silico evaluation and ADMET risk-codes generated from Admet predictor software used. Antimicrobial screening of compound 1 against selected enteropathogenic bacterial cells described showed MIC values in the range 57 μM - 114
μM while MBC values recorded were within the range 114 μM – 228 μM. The findings of this research identified trolline, isolated for the first time from M. jalapa as bioactive chemical entity present in methanol extract of aerial parts of M. jalapa.
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A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF DOCTOR OF PHILOSOPHY IN BIOCHEMISTRY DEPARTMENT OF BIOCHEMISTRY, FACULTY OF LIFE SCIENCES
AHMADU BELLO UNIVERSITY, ZARIA
NIGERIA
Keywords
ISOLATION AND CHARACTERIZATION,, ANTIBACTERIAL,, COMPOUNDS,, AERIAL PARTS,, CLAVILLIA,, (MIRABILIS JALAPA LINN,