PREPARATION AND EVALUATION OF A BIVALENT CLOSTRIDIUM PERFRINGENS TYPES C AND D VACCINE FOR CONTROL OF SHEEP ENTEROTOXAEMIA IN NIGERIA
PREPARATION AND EVALUATION OF A BIVALENT CLOSTRIDIUM PERFRINGENS TYPES C AND D VACCINE FOR CONTROL OF SHEEP ENTEROTOXAEMIA IN NIGERIA
| dc.contributor.author | AUDU, EMMANUEL ITODO | |
| dc.date.accessioned | 2015-01-27T08:46:31Z | |
| dc.date.available | 2015-01-27T08:46:31Z | |
| dc.date.issued | 1996 | |
| dc.description | A DISSERTATION SUBMITTED TO THE POSTGRADUATE SCHOOL AHMADU BELLO UNIVERSITY, ZARIA FOR THE DOCTOR OF PHILOSOPHY DEGREE IN THE DEPARTMENT OF VETERINARY PUBLIC HEALTH AND PREVENTIVE MEDICINE, FACULTY OF VETERINARY MEDICINE, AHMADU BELLO UNIVERSITY, ZARIA. 1996 | en_US |
| dc.description.abstract | Clostridium perfringens enterotoxaemia is the second most important clostridial disease after black quarter in Nigeria. There is no vaccine or vaccination programme to control it in Nigeria. In order to combat the disease problem, strains of CI. perfringens, types C and D, isolated in Nigeria, were used to prepare bivalent bacterins and toxoids in adjuvant systems (potash alum and sodium alginate) for vaccination of sheep. Five vaccines (bacterins and toxoids) viz: Anaculture (FC), a formalinized culture or bacterin; Anaculture + Potash alum (PaT); Anaculture + Sodium alginate (SaA); Toxoid + Potash alum (PaA); and Toxoid + Sodium alginate (SaT) were prepared. The vaccines were subjected to standard tests and used to inoculate sheep. The quality and quantity of the antibody response in sheep sera was determined by toxin-antitoxin neutralization tests in mice and the rocket immunoelectrophoresis methods. The efficacy and shelf-life of SaT were determined by challenge and field tests, and by periodical testing of the vaccine's (SaT, stored at 4°C and 24-26°C) ability to protect guinea pigs challenged with Clostridium perfringens toxins (beta and epsilon) respectively. The estimated beta (type C) and epsilon (type D) toxin content in reinforced clostridial medium culture of Cl. perfringens used for vaccine preparation was 2500 and 4000 units (mouse minimal lethal dose) per ml respectively. Antibody production as a result of vaccination with these vaccines was at its peak, 4 weeks post vaccination. Potash alum toxoid (PaT) produced the highest level (8.33+1.45 i.u. per ml) of beta-antitoxin,which | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/5879 | |
| dc.language.iso | en | en_US |
| dc.subject | PREPARATION, | en_US |
| dc.subject | EVALUATION, | en_US |
| dc.subject | BIVALENT, | en_US |
| dc.subject | CLOSTRIDIUM, | en_US |
| dc.subject | PERFRINGENS, | en_US |
| dc.subject | VACCINE, | en_US |
| dc.subject | SHEEP, | en_US |
| dc.subject | ENTEROTOXAEMIA | en_US |
| dc.title | PREPARATION AND EVALUATION OF A BIVALENT CLOSTRIDIUM PERFRINGENS TYPES C AND D VACCINE FOR CONTROL OF SHEEP ENTEROTOXAEMIA IN NIGERIA | en_US |
| dc.type | Thesis | en_US |