SYNERGISTIC ACTIVITY OF METHANOLIC EXTRACT OF ADENIUM OBESUM (APOCYNACEAE) STEM-BARK AND OXYTETRACYCLINE AGAINST SOME CLINICAL BACTERIAL ISOLATES
SYNERGISTIC ACTIVITY OF METHANOLIC EXTRACT OF ADENIUM OBESUM (APOCYNACEAE) STEM-BARK AND OXYTETRACYCLINE AGAINST SOME CLINICAL BACTERIAL ISOLATES
No Thumbnail Available
Date
2011
Authors
Tijjani, A.
M. S. Sallau,
Sunusi, I.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Adenium obesum plant (stem-bark) was collected, identified, air-dried, ground and extracted using
hot-continuous extraction method. Standard phytochemical tests and antimicrobial tests using
Kirby and Bauer techniques were adopted for the phytochemical screening and antimicrobial tests
of the plant stem-bark methanolic extract respectively. The MIC of methanolic extract in
combination with oxytetracycline using 8 different clinical bacterial isolates, both Gram positive
and Gram negative, was found to be around (62.5 pg/mi to 1250 pg/mi).In all, 87.5% showed
synergistic activity against 8 different bacterial isolates, both gram positive and gram negative
species. The highest synergistic activity was attained against Streptococcus pyogenes
.Phytochemical examination of the extract revealed the presence of alkaloids, steroids, saponins,
glycosides, anthraquinones, tannins and fiavonoids. This result indicated strong antibacterial
activity of the methanolic extract in combination with oxytetracycline against the pathogenic
bacterial isolates.
Description
Keywords
SYNERGISTIC,, METHANOLIC EXTRACT,, ADENIUM OBESUM,, STEM-BARK,, OXYTETRACYCLINE,, CLINICAL BACTERIAL ISOLATES