MONITORING OF ANTIBODIES AND ANTIGENS TO AFRICAN SWINE FEVER VIRUS (ASFV) IN PIGS FROM KADUNA AND PLATEAU STATES
MONITORING OF ANTIBODIES AND ANTIGENS TO AFRICAN SWINE FEVER VIRUS (ASFV) IN PIGS FROM KADUNA AND PLATEAU STATES
No Thumbnail Available
Date
2001-02
Authors
LUTHER, NANFWANG JORAM
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The prevalence rate of antibodies to African swine fever virus (ASFV) in parts of
Plateau and Kaduna states was investigated by indirect enzyme linked
immunosorbent assay (I-ELISA). Of a total of 590 pig sera tested from Plateau
state, 15 (2.5%) tested positive to ASFV antibodies. ASFV antibodies were
detected in serum samples from 3 out of the 4 local government areas of the
southern part of Kaduna state with Kaduna south local government area having
the highest rate of 29.4% and Jama'a having the lowest rate of 6%. None or the
serum samples from Kachia local government area had antibodies to ASFV. The
sex specific rate for male (7.4%) significantly differs from that of the female
(3.6%). (X2 = 5.721, P<0.05). The occurrence of antigens to ASFV in the study
area was determined by direct immunofluorescence (DIF). Nine out of 20 tissue
samples tested from Plateau state were positive for ASFV antigens while 5 out
of 6 tissue samples tested from Kaduna state were positive. Those tissues that
tested negative for ASFV antigens by DIF were retested for virus
deoxyribonucleic acid (DNA) by polymerase chain reaction (PCR). The results
showed that the 9 out of the 10 tissues tested were positive for virus DNA
genome except one tissue (i.e. 1 spleen). This study indicates the presence of
African swine fever (ASF) in Plateau and Kaduna states, with the conclusion that
ASF may be a major cause of mortality limiting pig production.
Description
A THESIS SUBMITTED TO POSTGRADUATE SCHOOL,
AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL
FULFILLMENT FOR THE DEGREE OF MASTER OF SCIENCE
(VETERINARY PATHOLOGY AND MICROBIOLOGY)
DEPARTMENT OF VETERINARY PATHOLOGY AND
MICROBIOLOGY, FACULTY OF VETERINARY MEDICINE,
AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA.
Keywords
ANTIBODIES,, ANTIGENS,, AFRICAN,, SWINE FEVER,, VIRUS,, PIGS,, KADUNA,, PLATEAU.