THE EFFECT OF CIGARETTE SMOKE MAINTAINED AT 10 – 32 PART PER MILLION OF CARBON MONOXIDE ON THE HIPPOCAMPUS OF WISTAR RATS
THE EFFECT OF CIGARETTE SMOKE MAINTAINED AT 10 – 32 PART PER MILLION OF CARBON MONOXIDE ON THE HIPPOCAMPUS OF WISTAR RATS
No Thumbnail Available
Date
2011-11
Authors
IBRAHIM, BADAMASI MOHAMMED
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Memory and Learning are diverse and complex entities attracting numerous studies with
different findings, some even contrasting each other. This study was conducted to make a
contribution towards unravelling this reality. Behavioural studies using the Morris Water
Maze task was elicited in Wistar rats which were grouped for weight and sex (nmale=18; n
female =12). Reference or latency time recordings for three (3) training at the start of the
experiment and after a 5week period of exposure to cigarette smoke (in case of exposed
Rats) or 5weeks training free period (in case of non-exposed Rats). Analysis of the time
recordings suggest that there is a significant decrease (p≤0.05)for the control male Wistar
rats unlike the insignificant(p≥0.05) change obtained for the control and experimental
female and experimental male. Similarly, assessment of the histological section of the
hippocampus of the Male Wistar rats from both tissue sections and hippocampal cell
suspension was done. Cell counting of normal and Pyknotic pyramidal cells was done using
Image J soft ware. Analysis of the cell count suggests a significantly higher (p≤0.05) count
in the Exposed Males only, for both the normal and pyknotic pyramidal cells compared to
the counts of the Control Male rats. In conclusion, male Wistar rats tend to retain Reference
memory of Morris Water Maze Task for a longer time (5 weeks in this case) compared to
female Wistar rats. Similarly, cigarette smoke maintained at 10-32ppm of Carbon Monoxide
for 4 days in a week for 5 weeks suffices in disrupting the memory capabilities of the Wistar
rats. With a corresponding level of higher rates of Pyknotic pyramidal cell.
Description
A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES,
AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILMENT OF THE
REQUIREMENT FOR THE AWARD OF A MASTERS OF SCIENCE (M.Sc.)
DEGREE IN HUMAN ANATOMY
DEPARTMENT OF HUMAN ANATOMY
FACULTY OF MEDICINE
AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA
Keywords
EFFECT,, CIGARETTE SMOKE,, AT 10 – 32 PART PER MILLION,, CARBON MONOXIDE,, HIPPOCAMPUS,, WISTAR RATS.