GASTROPROTECTIVE POTENTIAL OF AQUEOUS AND METHANOLIC LEAF EXTRACTS OF VITEX DONIANA ON ASPIRIN-INDUCED GASTRIC MUCOSAL DAMAGE AND SECRETIONS IN ALBINO RATS

Simple item page
dc.contributor.authorABANIWO, ROSE MAFO
dc.date.accessioned2016-08-08T08:43:35Z
dc.date.available2016-08-08T08:43:35Z
dc.date.issued2015-09
dc.descriptionA DISSERTATION SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF MASTERS DEGREE IN BIOCHEMISTRY DEPARTMENT OF BIOCHEMISTRY FACULTY OF SCIENCE AHMADU BELLO UNIVERSITY, ZARIA, NIGERIAen_US
dc.description.abstractThe study was designed to evaluate the gastro-protective activity of aqueous and methanolic extracts (250 mg/kg and 500 mg/kg) of V. doniana on aspirin-induced gastric mucosal damage in albino rats. A total of 49 adult male albino rats were used, which were randomly divided into 7 groups of 7 rats per group. Aqueous and Methanolic leaves extracts of V. doniana were administered for 14 days before induction of gastric mucosal damage. Ulcer index, preventive index, gastric volume, gastric pH, total acidity, gastric wall mucus and antioxidant activity of the plant extracts were determined using standard procedures. Both doses (250 mg/kg and 500 mg/kg) of aqueous extracts (9.25±0.96b, 9.00±2.28b) and methanolic extracts (4.43±1.72a, 8.50±1.73b) showed a significant (p<0.05) decrease in ulcer index when compared to the control group (0). The lower dose of both extracts showed significant higher preventive index when compared to the standard drug cimetidine (50 mg/kg). Total acidity and gastric juice volume in the aqueous (36.88±2.92b, 43.83±3.75c) (2.04±0.21a, 2.96±0.66b) and methanolic (32.70±2.61a, 38.58± 3.11b) (1.91±0.48a, 2.28±0.72ab)extract treated group showed a significant (p<0.05) decrease when compared to the control group (31.07±1.31a and 1.60±0.30a). There was no significant difference in the gastric pH of all the treated group when compared to the control. Gastric mucus significantly (p<0.05) decreased in the diseased group compared to normal control. There was a significant (P<0.05) decrease in Malondialdehyde (MDA) activity in both extract (25.58±0.74c, 28.17±0.39d)(17.47±0.87b, 24.94±0.15c)and a significant (p<0.05) increase in catalase activity (39.54±1.42a, 41.90±1.12a) (61.31±1.46c, 52.15±8.37b) in the extract treated group compared to the control group (11.14±0.79a and 68.49±1.75d). The histopathology of the stomach in the extract treated groupshowed slight and moderate ulceration when comparedto the control group. Chromatographic techniques were carried out on methanolic extract which showed the best gastro-protection. In-vitro antioxidant activity using 2,2-diphenyl 1-picryl ii hydrazyl (DPPH) scavenging activity was carried out on the different fractions obtained from column chromatography, fraction one which had the highest DPPH scavenging activity was further partially characterized by Fourier Transform Infrared Spectrophotometer (FTIR), the fraction was found to contain hydroxyl groups, halides, alcohols, phenols alkenes, carboxylic acid and alkane functional groups. Therefore the result suggests that the leaves of V. donianaposses gastro-protective potential and antioxidant properties justifying their use in folk medicine.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8368
dc.language.isoenen_US
dc.subjectGASTROPROTECTIVE POTENTIALen_US
dc.subjectAQUEOUS AND METHANOLIC LEAF EXTRACTSen_US
dc.subjectVITEX DONIANAen_US
dc.subjectASPIRIN-INDUCED GASTRICen_US
dc.subjectMUCOSAL DAMAGEen_US
dc.subjectSECRETIONS IN ALBINO RATSen_US
dc.titleGASTROPROTECTIVE POTENTIAL OF AQUEOUS AND METHANOLIC LEAF EXTRACTS OF VITEX DONIANA ON ASPIRIN-INDUCED GASTRIC MUCOSAL DAMAGE AND SECRETIONS IN ALBINO RATSen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
GASTROPROTECTIVE POTENTIAL OF AQUEOUS AND METHANOLIC LEAF EXTRACTS OF VITEX DONIANA ON ASPIRIN-INDUCED GASTRIC MUCOSAL DAMAGE AND SECRETIONS IN ALBINO RATS.pdf
Size:
1.43 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.58 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
SCIENCE