ISOLATION AND PARTIAL PURIFICATION OF LECTINS FROM THE STEM BARK OF ORDEAL TREE (Erythrophleum suaveolens) AND THEIR POSSIBLE LARVICIDAL ACTIVITIES AGAINST Culex quinquefasciatus

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dc.contributor.authorIHUOMA, NYEYIRICHI
dc.date.accessioned2014-02-10T10:49:09Z
dc.date.available2014-02-10T10:49:09Z
dc.date.issued2013-02
dc.descriptionA THESIS SUBMITTED TO THE POSTGRADUATE SCHOOL, AHMADU BELLO UNIVERSITY IN PARTIAL FULFILMENT OF THE REQUIREMENT FOR THE AWARD OF MASTER OF SCIENCE IN BIOCHEMISTRYen_US
dc.description.abstractABSTRACT Lectins from the saline extract of Erythrophleum suaveolens stem bark, were isolated, partially purified, characterized and evaluated in terms of their larvicidal properties against Culex quinquefasciatus. Purification was done by precipitation with ammonium sulfate at 20% - 90% saturation. The ammonium sulfate precipitate was subjected to gel filtration using Sephadex G-75 resin. The two fractions with the highest heamaglutinating activity were further subjected separately to ion-exchange chromatography using Sp- Sephadex C-50 resin. The lectins in the crude extract and the purified lectins appeared as five bands at positions 38KDa, 28KDa, 26KDa, 11KDa and 9KDa on a SDS-PAGE electrophoregram. The lectins were found to be stable at the temperature range of 30- 600C and pH range of 3-7. The carbohydrate content of the crude extract, Gel I, Gel II, Ion I and Ion II peaks were 0.04%, 0.04%, 0.03%, 0.02%, and 0.01% respectively and showed specificity to mannose and galactose. A series of nine concentrations of the extracts ranging from 0.0001 mg/L, 0.001 mg/L, 0.01 mg/L, 0.1 mg/L, 1mg/L, 5mg/L, 10 mg/L, 50 mg/L and 100mg/L were tested against the third instar larvae of Culex quinquefasciatus and their percentage mortalities and LC50 values were obtained. The crude extract of Erythrophleum suaveolens show very high larvicidal activity with 96% mortality achieved at a concentration of 100 mg/L and had an LC50 of 4.39 mg/L. The gel peaks of Erythrophleum suaveolens showed larvicidal activities to Culex quinquefasciatus larvae with 74.67% and 69.33% mortality achieved at a concentration of 100 mg/L and had LC50 of 43.73mg/L and 43.65mg/L respectively. The ion exchange purified extracts of Erythrophleum suaveolens showed low larvicidal activity to Culex quinquefasciatus larvae with 10.67% and 9.33% mortality achieved at a concentration of 100 mg/L respectively. These results suggest that the saline stem bark extract of Erythrophleum suaveolens is promising as a larvicide against mosquito larvae and can be used directly in small volumes in aquatic habitats and in mosquito population management programme. vien_US
dc.identifier.urihttp://hdl.handle.net/123456789/882
dc.language.isoenen_US
dc.subjectISOLATIONen_US
dc.subjectPARTIAL PURIFICATIONen_US
dc.subjectLECTINSen_US
dc.subjectSTEMen_US
dc.subjectBARKen_US
dc.subjectORDEAL TREE (Erythrophleum suaveolens)en_US
dc.subjectLARVICIDAL ACTIVITIESen_US
dc.subjectAGAINST Culex quinquefasciatusen_US
dc.titleISOLATION AND PARTIAL PURIFICATION OF LECTINS FROM THE STEM BARK OF ORDEAL TREE (Erythrophleum suaveolens) AND THEIR POSSIBLE LARVICIDAL ACTIVITIES AGAINST Culex quinquefasciatusen_US
dc.typeThesisen_US
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