DETECTION OF DENGUE AND YELLOW FEVER FLAVIVARIIDS IN AEDES (DIPTERA: CULICIDAE) POPULATIONS IN ZARIA AND ENVIRONS

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dc.contributor.authorIFRAIMU, DANIEL
dc.date.accessioned2015-10-21T08:04:19Z
dc.date.available2015-10-21T08:04:19Z
dc.date.issued2015-06
dc.descriptionA THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIAIN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF MASTER OF SCIENCE DEGREE IN ZOOLOGYen_US
dc.description.abstractDengue virus (DENV) and Yellow fever virus (YFV) are arboviruses transmitted by Aedes mosquitoes. The preponderance of Aedes mosquitoes in Zaria has been ascertained in some studies that reported on breeding of mosquitoes. However, the incidence of these viruses in the mosquito population is not known. This study investigated the incidence of the two arboviruses in the mosquito vectors in Zaria and its surroundings. Four locations were selected: Dumbi, Kufena, Hanwa and Bomo. Mosquitoes belonging to the genus Aedes were collected outdoor and indoor using mouth aspirators and pyrethroid-based insecticides knock-down method, from July to October 2013. Mosquitoes collected from the locations were pooled according to species, locations and whether they were collected outdoor or indoor. Viral RNA were extracted from the pools using RNA isolation kit (Shanghai ZJ Bio Technology). Real time reverse transcriptase polymerase chain reaction was used to detect dengue and yellow fever virus from the pools of mosquitoes. The target region for the four serotypes of dengue virus was the 3 UTR and the target region for yellow fever virus is the NS 5 gene. A total of 257 Aedes mosquitoes were collected. The result shows that Aedes vittatus(59.46%) was the most abundant species outdoor while Aedes aegypti(0.9%) was the least. Similarly, Aedes furcifer(50%) and Aedes aegypti(47.95%) were more preponderant indoor. Accordingly, Kufena (38.52%) had the highest collection of Aedes mosquitoes while Hanwa (17.12%) had the least collection. The fluorimeter channel (FAM) detected Ctvalues for 25 pool mosquito sample each for dengue and yellow fever virus. The Ct values ≤38 were positive as detected in the FAM. The Ct values show thatAedes vittatus from Dumbi, Hanwa and Kufena locations were negative for Dengue virus while Aedes furcifer, Aedes aegypti, Aedes taylori and Aedes metallicus were positive for Dengue virusin all the locations except in Kufena. Similarly, Aedes furcifer, Aedes aegypti, Aedes vittatus, Aedes taylori and Aedes metallicus were all positive for Yellow fever virus in all the locations. In all, 40%of the reaction samples were positive for Dengue virus while 60% were positive for Yellow fever virus from the four locations. Bomo recorded the highest incidence (60%) for Dengue virus detected in Aedes mosquitoes while Hanwa had the least record (10%). Similarly, Bomo had the highest incidence (33.33%) of Yellow fever virus while Dumbi and Hanwa each had the least incidence (20%). Based on the findings of this study, it is evident that dengue and yellow fever viruses are co-circulating in the Aedes population around Zaria and the mosquito vectors are active vectors in the four locations. In conclusion, this study has shown thatAedes furcifer and Aedes aegyptiare the most active vectors of Dengue and Yellow fever virus, whereas,Aedes vittatus is an active vector of Yellow fever in Kufena and Hanwa. This surveillance study has therefore brought to the fore, the urgent need for mosquito control in view of the emerging and re-emerging viral diseases.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/7037
dc.language.isoenen_US
dc.subjectDETECTIONen_US
dc.subjectDENGUEen_US
dc.subjectYELLOW FEVERen_US
dc.subjectFLAVIVARIIDSen_US
dc.subjectAEDESen_US
dc.subjectDIPTERA: CULICIDAE)en_US
dc.subjectPOPULATIONSen_US
dc.subjectZARIAen_US
dc.subjectENVIRONSen_US
dc.titleDETECTION OF DENGUE AND YELLOW FEVER FLAVIVARIIDS IN AEDES (DIPTERA: CULICIDAE) POPULATIONS IN ZARIA AND ENVIRONSen_US
dc.typeThesisen_US
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