MOLECULAR CHARACTERIZATION OF VANCOMYCIN-RESISTANT ENTEROCOCCUS SPP ISOLATED FROM PATIENTS AND HOSPITAL ENVIRONMENTS IN SOME HOSPITALS IN ABUJA, NIGERIA

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dc.contributor.authorNDUBUISI, JOHN CHIMA
dc.date.accessioned2018-09-25T14:04:27Z
dc.date.available2018-09-25T14:04:27Z
dc.date.issued2017-11
dc.descriptionA THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF DOCTOR OF PHILOSOPHY IN MICROBIOLOGY DEPARTMENT OF MICROBIOLOGY, FACULTY OF LIFE SCIENCES, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIAen_US
dc.description.abstractThis study investigated Enterococcus spp isolated from patients and hospital environment in University of Abuja Teaching Hospital, National Hospital, Kubwa and Kuje General Hospitals Abuja, Nigeria for vancomycin resistant enterococci. A total of 500 samples comprising stool, urine, wound swabs and environmental samples were analysed. The clinical samples yielded 97 enterococcal isolates while environmental samples yielded 5 enterococcal isolates giving a total of 102 isolates. The breakdown of the frequency of occurrence of the isolates indicate Enterococcus faecalis (n= 59; 57.8%), Enterococcus faecium (n=24;23.5%), Enterococcus casseliflavus (n=1;1.0%), Enterococcus gallinarum (n=3;2.9%), Enterococcus mundtii (n=11;10.8%), Enterococcus avium (n=1;1.0%), Enterococcus dispar 2(2.0%) and Enterococcus hirae (n=1;1.0%). They were identified by conventional, Microgen test kit and 16S rRNA sequencing methods. Antimicrobial susceptibility testing of the isolates was done using disk diffusion method. The strains that showed resistance to vancomycin were confirmed by Minimum Inhibitory Concentration (MIC) with E-test strips based on Clinical and Laboratory Standards Institute (CLSI) guidelines. Phenotypically, thirty four (34) of the isolates were vancomycin resistant and showed multi-resistance to teicoplanin, ampicillin, chloramphenicol, ciprofloxacin, rifampicin, erythromycin, doxycycline, linezolid, nitrofurantoin, streptomycin and gentimicin. Twelve strains were confirmed to be intermediate Vancomycin Resistant Enterococcus and possessed van genes detected by Polymarase Chain Reaction (PCR). Enterococcus mundtii, Enterococcus faecium and Enterococcus faecalis were less susceptible to antibiotics used than other isolated species. The results indicated that a significant percentage of isolates from clinical samples were resistant to different antibiotics used suggesting the need for improved control strategies to avoid dissemination of resistant isolates and emphasizing the importance of surveillance for the detection of emerging resistance traits.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/10546
dc.language.isoenen_US
dc.subjectMOLECULAR CHARACTERIZATION,en_US
dc.subjectVANCOMYCIN-RESISTANT ENTEROCOCCUS SPP ISOLATED,en_US
dc.subjectPATIENTS,en_US
dc.subjectHOSPITAL ENVIRONMENTS,en_US
dc.subjectHOSPITALS,en_US
dc.subjectABUJA,en_US
dc.subjectNIGERIAen_US
dc.titleMOLECULAR CHARACTERIZATION OF VANCOMYCIN-RESISTANT ENTEROCOCCUS SPP ISOLATED FROM PATIENTS AND HOSPITAL ENVIRONMENTS IN SOME HOSPITALS IN ABUJA, NIGERIAen_US
dc.typeThesisen_US
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