ISOLATION AND SCREENING OF STREPTOMYCES SPECIES FOR ENHANCED AMYLASE PRODUCTION

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dc.contributor.authorTHANIEL, Martina NA
dc.date.accessioned2018-06-20T10:46:07Z
dc.date.available2018-06-20T10:46:07Z
dc.date.issued2017-07
dc.descriptionADISSERTATION SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUREMENTS FOR THE AWARD OF A MASTER DEGREE IN MICROBIOLOGY DEPARTMENT OF MICROBIOLOGY, FACULTY OF LIFE SCIENCES, AHMADU BELLO UNIVERSITY, ZARIA NIGERIAen_US
dc.description.abstractIn this work, optimization of culture conditions for enhanced amylase production by five Streptomyces strains isolated from the agricultural soil from Ahmadu Bello University dam were investigated. Streptomyces isolates were identified by Gram staining, morphological and physiological observations and those identify were; Streptomyces narbonensis, Streptomyces michiganensis, Streptomyces orientalis, Streptomycesspp (S4), Streptomyces spp (S5). Upon screening for amylase production, only Streptomyces michiganensis was able to produce amylase with diameter of clear zones of starch hydrolysis of 38.3±0.45mm, hence it was used for further production of amylase under solid state fermentation. Determination of parameters such as period of fermentation, pH, temperature, moisture content and inoculum size were also carried out in this study. Highest amylase produced was observed at 72h incubation period with amylase activity of 8.5±21U/ml and 12.1±0.35U/ml using wheat bran and soluble starch respectively. The optimum period of fermentation, pH, temperature, initial moisture content and inoculum size were 48h (17.7±0.41U/ml), pH 6 (12.2±0.28U/ml), 35°C (11.2±1.13U/ml), 30% (7.9±0.21U/ml) and 2ml of 1.0 x106spores/ml (7.9±0.21U/ml) respectively. There was a significant increase in enzyme production after optimization of fermentation condition, with maximum yield of 17.7±0.41U/ml recorded at 48h.Wheat bran could serve as a good substrate for amylase production by Streptomyces michiganensis.en_US
dc.identifier.urihttps://kubanni.abu.edu.ng/handle/123456789/10005
dc.language.isoenen_US
dc.subjectISOLATION AND SCREENING,en_US
dc.subjectSTREPTOMYCES SPECIES,en_US
dc.subjectENHANCED AMYLASE,en_US
dc.subjectPRODUCTION,en_US
dc.titleISOLATION AND SCREENING OF STREPTOMYCES SPECIES FOR ENHANCED AMYLASE PRODUCTIONen_US
dc.typeThesisen_US
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