PHYTOCHEMICAL SCREENING AND ANTIFUNGAL EFFECTS OF EUPHORBIA HETEROPHYLLA LINN. AND MITRACARPUS SCABER ZUCC. EXTRACTS
PHYTOCHEMICAL SCREENING AND ANTIFUNGAL EFFECTS OF EUPHORBIA HETEROPHYLLA LINN. AND MITRACARPUS SCABER ZUCC. EXTRACTS
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Date
2016-10
Authors
SHITTU, Michael Adeniyi
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Abstract
The whole plant extracts of Euphorbia heterophylla and Mitracarpus scaber were screened for their phytochemical properties and antifungal effects. Aqueous, methanol, ethyl acetate and n-hexane were used as solvents for extraction of the plant samples. The plant samples were also screened qualitatively and quantitatively for their bioactive constituents. The aqueous, methanol, ethyl acetate and n- hexane extracts of the whole plant of Euphorbia heterophylla and M. scaber were concentrated at 100mg/ml, 50mg/ml, 25mg/ml and 12.5mg/ml respectively. The antifungi activities of the plant samples were tested against Candida albicans, Trichophyton mentagrophytes, Microsporum auduounii and Aspergillus flavus. The MIC and MFC of the extracts was also determined for all the fungi species. The result revealed that there were no significant differences in bioactive constituents of both plants qualitatively and quantitatively. Ethyl acetate extract of Euphorbia heterophylla and Mitracarpus scaber had highest zone of inhibition of 24.50±0.71mm and 26.00±0.00mm on Candida albicans, 24.00±0.00mm and 24.00±0.00mm on Trichophyton mentagrophytes while aqueous extract inhibition was 20.00±.00mm and 22.00±0.00mm on Candida albicans, 18.00±0.00mm and 18.00±0.00mm on Trichophyton mentagrophytes respectively. No zone of inhibition was produced in Microsporum auduouinii and A. flavus in all the solvent extracts used. The combined effects of Euphorbia heterophylla and Mitracarpus scaber plant extracts using the same solvents (aqueous, methanol, ethyl acetate and n–hexane) showed significant differences in all the solvent extracts at 100mg/ml of aqueous (23.00±0.00mm and 20.00±0.00mm), methanol (19.00±0.00mm and 18.00±0.00mm), ethyl acetate (28.00±0.00mm and 26.00±0.00mm) and n-hexane (20.00±0.00mm and 17.00±0.00mm) on Candida albicans and Trichophyton mentagrophytes respectively. No zone of inhibition was shown in Aspergillus flavus and Microsporum auduouinii. The MIC and MFC ranged from 50 – 6.25mg (MIC) and 100 – 6.25mg (MFC). Thus the traditional claims of the uses of the plants as antifungal agents were therefore justified.
Description
A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES AHMADU BELLO UNIVERSITY ZARIA IN PARTIAL FULFILLMENT OF REQUIREMENTS FOR THE AWARD OF MASTER DEGREE IN EDUCATIONAL BIOLOGY
DEPARTMENT OF BIOLOGICAL SCIENCES,
FACULTY OF SCIENCE.
AHMADU BELLO UNIVERSITY, ZARIA,
NIGERIA
Keywords
PHYTOCHEMICAL SCREENING,, ANTIFUNGAL EFFECTS,, EUPHORBIA HETEROPHYLLA LINN,, MITRACARPUS SCABER ZUCC,, EXTRACTS