EVALUATION OF CORNBRAN ('DUSA'), YAM AND BANANA PEELS AS POSSIBLE SUBSTRATES FOR AMYLASE PRODUCTION BY LOCAL STRAINS OF ASPERGILLUS NIGER CA-3 AND OR-5
EVALUATION OF CORNBRAN ('DUSA'), YAM AND BANANA PEELS AS POSSIBLE SUBSTRATES FOR AMYLASE PRODUCTION BY LOCAL STRAINS OF ASPERGILLUS NIGER CA-3 AND OR-5
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Date
1989
Authors
ADO, SALEH ALHAJI
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Abstract
A total of forty five strains of Aspergillus niger were
isolated from various sources and screened for amylase
producing ability using Nutrient agar containing soluble
starch as the substrate. A. niger CA-3 and OR-5 were selected
as the highest amylase producers. The two selected strains
were studied further for amylase production in submerged
cultures using corn bran ('Dusa'), yam and banana peels as the
substrates with soluble starch as the control. When grown on
soluble starch, the highest amylase activity for A. niger CA-3
was 24 units/ml while it was 27, 297 and 150 units/ml when
grown on corn bran ('Dusa'), yam and banana peels
respectively. Similarly, the highest amylase activities in the
culture filterates of A. niger OR-5 grown on soluble starch,
corn bran ('Dusa'), yam and banana peels were 36, 66, 222 and
144 units/ml respectively. The optimum concentration of yam
peel powder for amylase production by A. niger CA-3 was 3.5%
(w/v), while the optimum pH and temperature were 7.0 and 30°C
respectively. It was concluded that yam peel was the best, and
banana peel and corn bran ('Dusa') are also better substrates
for amylase production by both strains than soluble starch.
Description
A THESIS SUBMITTED TO THE POSTGRADUATE SCHOOL AHMADU BELLO
UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS
FOR THE DEGREE OF MASTER OF SCIENCE
DEPARTMENT OF MICROBIOLOGY
FACULTY OF SCIENCE
AHMADU BELLO UNIVERSITY
ZARIA, NIGERIA
Keywords
EVALUATION, CORNBRAN, ('DUSA'), YAM, BANANA, PEELS, POSSIBLE, SUBSTRATES, AMYLASE, PRODUCTION, LOCAL, STRAINS, ASPERGILLUS, NIGER, CA-3, -5