EFFECTS OF Aspergillus niger AND Trichoderma viride ON THE NUTRIENT COMPOSITION OF GROUNDNUT HUSK
EFFECTS OF Aspergillus niger AND Trichoderma viride ON THE NUTRIENT COMPOSITION OF GROUNDNUT HUSK
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Date
2014-08
Authors
ALIYU, Nafisatu
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Abstract
The effects of Aspergillus niger and Trichoderma viride on the nutritional composition of pre-treated groundnut husk (GH) with Ustilago maydis spores (biological, BGH) and 2M NaOH (chemical, CGH) were studied. The pre-treated groundnut husk were subjected to monoculture, co-culture and crude enzyme extract fermentation using A. niger and T. viride so as to evaluate its lignin and cellulose contents, quantify cellulase activities; determine proximate, anti-nutrient, mineral and amino acid contents. Enzymatic activities in all the pretreated GH increased steadily from day one and peaked off at day seven. The biological pretreated GH showed significant (p<0.05) increase in total cellulase and endoglucanase yield (activity) than the alkali pretreated GH. The proximate analysis of the cellulosic residues differed from one treatment to another, with chemical pretreated GH on which T. viride (CGHT. viride) was inoculated being the best as a result of 73% increase in protein with significant (p<0.05) decrease in anti-nutritional content; phytate (51.22g/100g) and alkaloid (7g/100g). Significantly (p<0.05) higher values of phosphorous, zinc and magnesium were observed for all fermented GH compared to untreated GH (control), while significantly (p<0.05) lower values of copper, sodium and iron were observed for all fermented GH compared to control. Except for aspartate, there was general decrease in amino acid content of all pretreated GH compared to the standard feed protein used to standardize the Amino acid analyser. Even though, the amino acid content of CGHA. niger were slightly higher than CGHT. viride. The results of this study indicate that T. viride is a potentially viable microorganism for the production of enriched and safe animal feed.
Description
A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENT FOR THE AWARD OF MASTER OF SCIENCE (M. Sc) DEGREE IN BIOCHEMISTRY. DEPARTMENT OF BIOCHEMISTRY, FACULTY OF SCIENCE, AHMADU BELLO UNIVERSITY, ZARIA NIGERIA. AUGUST, 2014.
Keywords
EFFECTS,, Aspergillus niger,, Trichoderma viride,, NUTRIENT COMPOSITION,, GROUNDNUT HUSK.