EXTRACTION AND PURIFICATION OF SIALIC ACID FROM SUBMANDIBULAR MUCIN OF CATTLE AND GOAT
EXTRACTION AND PURIFICATION OF SIALIC ACID FROM SUBMANDIBULAR MUCIN OF CATTLE AND GOAT
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Date
2014-06
Authors
NKEONYE, OGECHI LYNDA
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Abstract
In this study, Sialic acid (Neuraminic acid) was extracted, purified and partially characterized
from submandibular mucin of cattle and goat. The extraction involved homogenization,
treatment with enzymes (phospholipase and lipase) and acid hydrolysis. Ion exchange
chromatography was used to purify the sialic acids. The partial characterization involved thinlayer
chromatography and Fourier transform infrared spectroscopy (FTIR). Treatment of the
submandibular mucin with phospholipase led to an increase in release of sialic acid with
optimum release of sialic acids at 90 minutes while treatment of the homogenate with lipase led
to a slight increase in sialic acids released. Acid hydrolysis of the homogenized submandibular
glands led to a 15 fold increase in sialic acids released. The purification fold after carrying out
ion exchange chromatography on the sialic acid extracts of the cattle and goat submandibular
mucin was 16.59 and 12.11 respectively. Thin-layer chromatography revealed that both sialic
acid extracts moved at the same pace with a standard sialic acid from Sigma. The FTIR spectra
of the purified sialic acid and that of the standard sialic acid had wavenumbers that are indicative
of alkyl halide, alkenes, allene, alkane and carboxylic acid which are the functional groups that
are found in sialic acids. The results indicated that the sialic acid contained in goat
submandibular gland is more and of similar quality with that from cattle submandibular gland.
The results have indicated that sialic acids are extractible from the submandibular mucins with
the aid of phospholipase and lipase.
Description
A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES,
AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA IN PARTIAL FULFILMENT OF
THE REQUIREMENTS FOR THE AWARD OF MASTER OF SCIENCE IN
BIOCHEMISTRY
DEPARTMENT OF BIOCHEMISTRY
FACULTY OF SCIENCE
AHMADU BELLO UNIVERSITY
ZARIA
NIGERIA
JUNE, 2014
Keywords
EXTRACTION,, PURIFICATION,, SIALIC,, ACID,, SUBMANDIBULAR,, MUCIN,, CATTLE,, GOAT