DEVELOPMENT OF ROUTINE CULTURE MEDIA FROM SORGHUM
DEVELOPMENT OF ROUTINE CULTURE MEDIA FROM SORGHUM
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Date
1997-02
Authors
IZEBE, KASIMU SALIHU
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Abstract
ABSTRACT
The nutritive value of sorghum grains (Short Kaura(SK)
variety) was explored in the development of culture media.
The sorghum (SK) starch and proteineous materials were extracted,
dried and subjected to amylase and pepsin hydrrolysis respectively.
Dried powder each of bean husk, soybean, spent yeast and fish was
treated with pepsin hydrolysis to obtained growth supplements.
Sorghum starch, protein digests and growth supplements were
reconstituted into varied media were used in liquid and solid
foems to grow Staphylococcus aureus (NCTC 6571), Bacillus subtilis
(NCTC 8241), Escherichia coli (NCTC 10418), Pseudomonas aeruginosa
(NCTC 6750) and Candida albicans (ATCC 10231). The growth of
these micro-organisms were better in commercially prepared Nutrient
broth than the sorghum broth. 20% (0.l03g/L glucose) sorghum broth
gave the best growth and 40% (0.206g/L glucose) sorghum broth was
the least.
Sorghum broths with growth supplements performed better in
supporting the growth of the test micro-organisms than the
commercially prepared Nutrient broth. 35% (3.78/L protein) fish
suppllement in sorghum broth gave better growth than the other
formulations. On solidifying the formullated sorghum media with
one percent agar, the resultant media also proved better in
supporting the growth of the test micro-organisms than the
commercially prepared Nutrient agar.
Description
A THESIS SUBMITTED TO THE POSTGRADUATE SCHOOL,
AHMADU BELLO UNIVERSITY, ZARIA,
IN PARTIAL FULFILLMENT OF THE REQUIREMENTS
FOR THE AWARD OF THE DEGREE OF MASTER OF
Keywords
DEVELOPMENT, ROUTINE, CULTURE MEDIA, FROM SORGHUM