IN VITRO FREE RADICAL SCAVENGING AND DNA PROTECTING ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS OF LEAF AND STEM BARK OF Gossypium barbadense
IN VITRO FREE RADICAL SCAVENGING AND DNA PROTECTING ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS OF LEAF AND STEM BARK OF Gossypium barbadense
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Date
2016-11
Authors
ADEOLA MARY, ADEWUSI
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Abstract
Prevention and treatment of various degenerative diseases using traditional medicines is
increasingly generating interest, especially in clinical research. This study was aimed to evaluate
the invitro free-radical scavenging properties and potential to prevent DNA damage (from
oxidative stress) of leaf and stem bark extracts of Gossypium barbadense. Quantitative and
Qualitative phytochemical test of different solvent extracts (hexane extract, ethyl acetate extract
and aqueous extract) was carried out and result showed that hexane leaf extract had the highest
amount of flavonoids (1734.54 ± 6.36 µg equivalent Quercertin/g dry material) and ethyl acetate
leaf extract has the highest phenolic content (86.04 ± 0.85 µg Gallic acid/g of dry sample).
Scavenging of DPPH radical by all the extracts was evaluated to estimate their antioxidant
potential. All the extracts possess free radical scavenging property with hexane leaf extract
having the lowest IC50 value of 0.192 ± 0.076 mg/ml; as compared to the standard, ascorbic acid
with IC50 value of 0.173 ± 0.098 µg/ml. The IC50 value is in the increasing order of HL > EL >
ES > HS > AqL > AqS. Protection of DNA against free radicals-induced oxidative damage (free
radicals from Fentons reagent and photolyzed H2O2) by all extracts was studied using Pmax
Green Florescence Protein (GFP) plasmid. The result showed that only the aqueous leaf extract
had DNA protecting activity. Membrane stabilization test – a measure of anti-inflammatory
activity was also carried out. It was observed that all the extracts protected the RBC from
hemolysis as compared with a standard, aspirin.
Description
IN PARTIAL FULFILLMENT FOR THE AWARD OF MASTER OF SCIENCE IN
BIOCHEMISTRY
DEPARTMENT OF BIOCHEMISTRY
FACULTY OF SCIENCE
AHMADU BELLO UNIVERSITY,
ZARIA, NIGERIA
Keywords
IN VITRO FREE RADICAL SCAVENGING,, DNA PROTECTING ACTIVITIES,, DIFFERENT SOLVENT EXTRACTS,, LEAF AND STEM BARK OF Gossypium barbadense,