LABORATORY CULTURE OF SOME SPECIES OF MUSHROOMS COLLECTED IN AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA

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Date
1996-06
Authors
NWANZE, PETER IFEANYI
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Abstract
Studies on spore germination of 15 mushroom species collected from various areas in Ahmadu Bello University, Zaria were carried out on potato dextrose, Sabouraud's dextrose, and yeast extract agar. Each of them was supplemented with peptone, malt extract, yeast extract, spawn, and charcoal in 32 different combinations. Out of 15 species only the spores of Lentinus squarrosulus (Mont.)Singer consistently germinated in all the media. Attempt was also made to culture the 15 mushroom species from the hyphae collected from the pileus on 4 different hyphal culture media. Only L. sauarrosulus grew in all different media while Termitomyces clypeatus Heim. only grew in the media which contained thiamine hydrochloride. Carpogenesis was studied in L. squarrosulus using two composted media and five non-composted media. Carpogenesis successfully occurred in 4 out of the 5 non-composted media while carpogenesis failed to occur in any composted media. Fruiting was induced by direct casing of spawn, casing of some non-composted media and traumatizaton of hyphae. Only the spawned sawdust media cased with sterilized soil to which activated charcoal had been added produced fruit while both the cased spawn and the traumatized hyphae produced negative results. The study shows that it is possible to effectively culture L. squarrosulus for laboratory purposes within 25 days. It also confirmed that this can easily be done using locally sourced materials.
Description
A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF THE DEGREE OF MASTER OF SCIENCE (M.SC EDUC. BIOLOGY) DEPARTMENT OF BIOLOGICAL SCIENCES AHMADU BELLO UNIVERSITY, ZARIA JUNE, 1996
Keywords
LABORATORY,, CULTURE,, SPECIES,, MUSHROOMS,, COLLECTED,, AHMADU, BELLO,, UNIVERSITY,, ZARIA,, NIGERIA.
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