LABORATORY CULTURE OF SOME SPECIES OF MUSHROOMS COLLECTED IN AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA
LABORATORY CULTURE OF SOME SPECIES OF MUSHROOMS COLLECTED IN AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA
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Date
1996-06
Authors
NWANZE, PETER IFEANYI
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Abstract
Studies on spore germination of 15 mushroom species
collected from various areas in Ahmadu Bello University, Zaria
were carried out on potato dextrose, Sabouraud's dextrose, and
yeast extract agar. Each of them was supplemented with
peptone, malt extract, yeast extract, spawn, and charcoal in
32 different combinations. Out of 15 species only the spores
of Lentinus squarrosulus (Mont.)Singer consistently
germinated in all the media. Attempt was also made to culture
the 15 mushroom species from the hyphae collected from the
pileus on 4 different hyphal culture media. Only L.
sauarrosulus grew in all different media while Termitomyces
clypeatus Heim. only grew in the media which contained
thiamine hydrochloride.
Carpogenesis was studied in L. squarrosulus using two
composted media and five non-composted media. Carpogenesis
successfully occurred in 4 out of the 5 non-composted media
while carpogenesis failed to occur in any composted media.
Fruiting was induced by direct casing of spawn, casing of
some non-composted media and traumatizaton of hyphae. Only the
spawned sawdust media cased with sterilized soil to which
activated charcoal had been added produced fruit while both
the cased spawn and the traumatized hyphae produced negative
results.
The study shows that it is possible to effectively
culture L. squarrosulus for laboratory purposes within 25
days. It also confirmed that this can easily be done using
locally sourced materials.
Description
A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE
REQUIREMENTS FOR THE AWARD OF THE DEGREE OF
MASTER OF SCIENCE (M.SC EDUC. BIOLOGY)
DEPARTMENT OF BIOLOGICAL SCIENCES
AHMADU BELLO UNIVERSITY, ZARIA
JUNE, 1996
Keywords
LABORATORY,, CULTURE,, SPECIES,, MUSHROOMS,, COLLECTED,, AHMADU, BELLO,, UNIVERSITY,, ZARIA,, NIGERIA.