SEARCH FOR E. COLI O157:H7 AND SALMONELLA SPECIES IN WATER SAMPLES AND THEIR ANTIBIOTIC PROFILES FROM SELECTED WELLS IN KATSINA STATE, NIGERIA

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dc.contributor.authorMUKHTAR, Fatima
dc.date.accessioned2018-08-09T10:02:54Z
dc.date.available2018-08-09T10:02:54Z
dc.date.issued2016-06
dc.descriptionA DISSERTATION SUBMITTED TO THE SCHOOL OF POST GRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA, IN PARTIAL FULFILLMENT FOR THE AWARD OF A MASTER DEGREE IN MICROBIOLOGY DEPARTMENT OF MICROBIOLOGY, FACULTY OF SCIENCE, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIAen_US
dc.description.abstractThe lack of clean water resources and sanitation facilities looms as one of the most serious environmental health problems faced by a large fraction of the world’s population. The onset of water borne diseases is enormous and largely attributed by the fact that, the World Health Organisation (WHO) has estimated that 1.1 billion people globally lack basic access to drinking water resources,while 2.4 billion have inadequate sanitation facilities which accounts for many water related acute and chronic diseases. The research hyphothesis is that, E.coli O157:H7 and Salmonella spp are more likely to be found in wells with close proximity to pit laterines. The study was designed to search for the presence of Escherichia coli O157:H7 and Salmonella spp from wells in Dan-musa, Mani and Danja Local Government Areas of Katsina State. The antibiotics susceptibility profiles of the isolates was also carried out. Some physicochemical parameters (temperature, pH, turbidity and odour), and also distance of wells to pit laterines were determined at collection points. The organisms were isolated by cultural methods using selective media and were subjected to series of biochemical tests. Salmonella isolates were sero-grouped using seroquick grouping reagents. Antibiotic susceptibility testing was performed by disk diffusion method. The physicochemical parameters analysed were within the WHO range but all the distances measured were below the set standard of 30 meters by WHO and the Nigerian Environmental Protection Agency. Dan-musa Local Government Area had the highest mean coliform and aerobic plate counts of 20.7cfu/ml and 3.47±0.02 respectively, while Danja had least mean coliform and aerobic plate count of 13.8cfu/ml and 1.99±0.02. Out of the 300 well water samples collected, a total of 246 presumptive E.coli isolates were isolated and 7 out of them were serologically confirmed to be E.coli O157:H7, also a total of 11 Salmonella strains were isolated belonging to three(3) different sero-groups (Group D, A and B). Significant ix statistical association was found between the occurrence of these bacterial isolates and distance of wells to pit laterines. All the isolates were resistant to multiple classes of antibiotics, the highest resistance of 100% was to tetracycline and augmentin by E.coli O157:H7, while for Salmonella species, highest resistance was to ceftazidime and cefuroxime, 100% each. However, the two organisms were sensitive to flouroquinolones and all the Salmonella isolates were susceptible to chloramphenicol and cefoxitin. The presence of these pathogenic bacteria from the well water samples analysed connote a serious health risk to consumers due to threat of disease and antibiotic resistance. This study accenuates the need to set standards for citing of wells away from pit laterines in the study locations, Provision of adequate potable water, improved sanitation and restricting the use of drugs either for self-medication and as growth promoters in food animals were recommended to overcome the problems of multi-drug resistant organisms.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/10031
dc.language.isoenen_US
dc.subjectSEARCH,en_US
dc.subjectE. COLI O157,en_US
dc.subjectH7,en_US
dc.subjectSALMONELLA SPECIES,en_US
dc.subjectWATER SAMPLES,en_US
dc.subjectANTIBIOTIC PROFILES,en_US
dc.subjectSELECTED WELLS,en_US
dc.subjectKATSINA STATE,en_US
dc.subjectNIGERIA,en_US
dc.titleSEARCH FOR E. COLI O157:H7 AND SALMONELLA SPECIES IN WATER SAMPLES AND THEIR ANTIBIOTIC PROFILES FROM SELECTED WELLS IN KATSINA STATE, NIGERIAen_US
dc.typeThesisen_US
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