PREVENTIVE EFFECT OF DIETARY CUCURBITA MAXIMA DUCH FRUIT INCLUSION ON N- METHYL-N-NITROSOUREA (MNU) INDUCED COLON CARCINOGENESIS IN MALE WISTAR RATS
PREVENTIVE EFFECT OF DIETARY CUCURBITA MAXIMA DUCH FRUIT INCLUSION ON N- METHYL-N-NITROSOUREA (MNU) INDUCED COLON CARCINOGENESIS IN MALE WISTAR RATS
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Date
2017-12
Authors
ISIAKA, IBRAHIM
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Abstract
The preventive effect of dietary Cucurbita maxima fruit inclusion at 2.5%, 5% and 10% on colon carcinogenesis initiated with N-methyl-N-nitrosourea (MNU) induction for twelve (12) weeks was evaluated. Group A= Normal saline with standard feed, Group B= MNU with standard feed, Group C= MNU with dietary inclusion of 2.5% Cucurbita maxima powder, Group D= MNU with dietary inclusion of 5% Cucurbita maxima powder, Group E= MNU with dietary inclusion of 10% Cucurbita maxima powder, Group F= Dietary inclusion control normal saline with 10% Cucurbita maxima powder. After twelve (12) weeks of treatment, the colon and liver of rats undergoing MNU intoxication but fed Cucurbita maxima fruit included diets showed a significant (p<0.05) increase in the activity of endogenous antioxidant enzymes (catalase and superoxide dismutase) when compared with MNU control group. Also a dose-dependent decrease in the extent of lipid peroxidation was observed in the colon and liver of dietary Cucurbita maxima fruit included treated groups. A significant (p<0.05) increase in white blood cell, platelet level, neutrophils and lymphocyte counts in MNU control group was observed when compared to other groups fed with dietary Cucurbita maxima fruit inclusion, while a significant (p<0.05) decrease in packed cell volume, haemoglobin count and red blood cell were observed in MNU control group compared to other groups fed with dietary Cucurbia maxima fruit inclusion. Histological observation of colon and liver tissue sections showed severe focal area of inflammatory cells in the MNU control group while normal control group and other groups that received various dietary Cucurbita maxima fruit inclusion showed milder to no tissue damage. A statistically significant (p<0.05) decrease in carcino-embrayonic antigen (CEA) levels was observsed in all dietary Cucurbita maxima fruit inclusion groups compared to MNU control group. Immunohistochemical observations of the colon tissue sections showed no expression of
Mut L Homolog gene in MNU control group while moderate to mild expression of Mut L Homolog gene in normal control group and other groups treated with 2.5%, 5% and 10% of dietary Cucurbita maxima fruit inclusion. These results indicate potential of dietary Cucurbita maxima fruit inclusion to antagonizing the process of chemically induced colon carcinogenesis by enhancement of endogenous antioxidants systems and prevention of oxidative stress.
Description
A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES,
AHMADU BELLO UNIVERSITY, ZARIA
IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD
OF
MASTERS DEGREE IN NUTRITION.
DEPARTMENT OF BIOCHEMISTRY
FACULTY OF LIFE SCIENCES
AHMADU BELLO UNIVERSITY, ZARIA
NIGERIA
Keywords
PREVENTIVE EFFECT,, DIETARY CUCURBITA MAXIMA,, DUCH FRUIT INCLUSION,, N- METHYL-N-NITROSOUREA,, INDUCED COLON CARCINOGENESIS,, MALE WISTAR RATS