CHANGES IN THE LEVELS OF SIALIC ACID AMONG ORGANS DURING THE COURSE OF STREPTOZOTOCIN-INDUCED TYPE I AND TYPE II DIABETES
CHANGES IN THE LEVELS OF SIALIC ACID AMONG ORGANS DURING THE COURSE OF STREPTOZOTOCIN-INDUCED TYPE I AND TYPE II DIABETES
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Date
2016-09
Authors
OBOCHI, PETER
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Abstract
This study was undertaken to investigate the changes of sialic acid levels in some tissues: liver, kidney, pancreas, skeletal muscle and brain of sreptozotocin-induced diabetic rats over a period of 9 weeks and 8 weeks respectively for types I and II so as to understand the actual source of serum sialic acid upsurge during diabetes. The animals were classified into type I diabetic group (TIDG), type II diabetic group (TIIDG) and the normal control group (NCG). The type I diabetic condition was induced using 60 mg/kg body weight of streptozotocin while the type II diabetes was induced with 10% fructose along with 40 mg/kg body weight of streptozotocin. Fasting blood glucose, free serum sialic acid level and total sialic acid level in the tissues were monitored at 3 and 2 weeks intervals for a period of 9 and 8 weeks for type I and type II diabetes, respectively. There was a significant (P<0.05) decrease in free serum sialic acid level at the early stage at (week 2) for the TIIDG. The same pattern was observed in the TIDG at week 3 but insignificant (P>0.05). Also, there was an increase in free serum sialic acid level with the progression of the disease in TIDG and TIIDG, though insignificant (P>0.05) compared to the NCG. There was a significant (P<0.05) increase in total sialic acid level in the following tissues; brain, kidney, pancreas, skeletal muscle except the liver. Correlation analysis performed on the relation between levels of sialic acids in the serum and tissues shows that there was a level of varying negative correlation between the level of free serum sialic acids and the total sialic acid in the entire tissues; liver, kidney, pancreas, skeletal muscle and brain over the course of the experiment. The correlations show a relationship of increasing level of serum free sialic acid with decreasing total sialic in the tissues. This pattern of relationship, suggests that sialic acids in the serum were possibly liberated from the tested individual tissues. Increase in sialic acids in these tissues: liver, kidney, pancreas, skeletal muscle and brain is responsible for increased level free serum sialic acids seen in diabetes mellitus.
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A DISSERTATION SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA
IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD
OF A
MASTER OF SCIENCE DEGREE IN BIOCHEMISTRY
DEPARTMENT OF BIOCHEMISTRY
FACULTY OF SCIENCE
AHMADU BELLO UNIVERSITY,
ZARIA, NIGERIA
Keywords
CHANGES,, LEVELS,, SIALIC ACID,, ORGANS,, COURSE,, STREPTOZOTOCIN-INDUCED TYPE I,, TYPE II DIABETES,