THE EFFECT OF 4-NITROQUINOLINE 1-OXIDE ON THE UPTAKE AND INCORPORATION OF SELECTED AMINO ACIDS BY LACTOBACILLUS CASEI

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dc.contributor.authorBarker, Charles Lawrence
dc.date.accessioned2020-02-26T09:32:55Z
dc.date.available2020-02-26T09:32:55Z
dc.date.issued1973-05
dc.descriptionA Dissertation Submitted to the Faculty of Baylor University In Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophyen_US
dc.description.abstractUtilizing Lactobacilling casei cells an experimental teat system was developed to measure the effect of varying concentrations of the potent, water-soluble, oncogen 4-nitroquinoline 1-oxide (4-4Q0) on amino acid uptake and Incorporation by the bacteria. The rates of uptake, incorporation, and removal of tritium labeled 4-NQ0 were measured. Clycine-14C, L-alauine-14C, L-methionine-14C, and L-tyrosine-14C standard uptake and Incorporration measurements wore also taken. The labeled aaiino acids all displayed a rapid Initial uptake with saturation being almost completed after 180 seconds. The 4-UQ0-3H uptake was initially rapid, reaching a maximum limit after approximately 60 seconds, after which an equally rapid renoval of tritium label was experienced. After 180 seconds from the time of oncogen introduction the bacteria had returned to a bound oncogen label approximately one sixth of the maximum level. The standard incorporated amino acid curves showed a time dependency with maximum levels being reached after 10 minutes. Incorporated oncogen showed an initial level that was approximately twice as high as the level measured after 180 or 600 seconds. Of the amino acids tested L-alanine-14C was the most Sensitive to 4-NQ0 levels. When C.0167 mM 4-NQ0 was introduced Into the test system reduction of L-alanine-14C uptake was noted. Incorporation of the same amino acid was stimulated. In general, the same results were shown for the other amino acids though they we re less sensitive to the presence of oncogen. An alteration in the amino acid incorporation curves, deaonst rating an apparent dependence on the amino acid concentration in the cell and not on time , was noted in all of the data. From the data presented a mechanists for the alteration of biological systems by 4-nitroquinollne l-oxide was given. The reasoning suggests that an alteration of the messenger ribonucleic acid may be the point of initial metabolic change. Other known oncogens we re also introduced into the experimental system. They were N-cicrosodimethylamine and X-nethyl-N'nitro-N'-nitrosoguanidine. Both chemicals induced a simllar aIteration of the standard L-alanine-14C incorporation demonstrated by 4-NQ0. These data suggest that this system may be used to screen potential oncogens.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/12238
dc.language.isoenen_US
dc.subjectEFFECT OF 4-NITR0QUIN0LINE 1-OXIDE,en_US
dc.subjectUPTAKE AND INCORPORATION,en_US
dc.subjectSELECTED AMINO ACIDS,en_US
dc.subjectLACTOBACILLUS CASEIen_US
dc.titleTHE EFFECT OF 4-NITROQUINOLINE 1-OXIDE ON THE UPTAKE AND INCORPORATION OF SELECTED AMINO ACIDS BY LACTOBACILLUS CASEIen_US
dc.typeThesisen_US
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