KINETIC STUDY OF PINEAPPLE (Ananas comosus) PEEL CONVERSION TO GLUCOSE BY Aspergillus niger
KINETIC STUDY OF PINEAPPLE (Ananas comosus) PEEL CONVERSION TO GLUCOSE BY Aspergillus niger
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Date
2012-12
Authors
AKINOLA PHILIP, OSOSANYA,
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Abstract
The kinetic of the production of glucose from pineapple using Aspergillus niger species isolated
from fresh groundnut seed has been investigated. Soluble sugar was extracted from the pineapple
peel, the peel was then dilignified and subsequently enzymatic hydrolysis of the polysaccharides
to glucose was conducted. Effect of the operational parameters such as enzyme loading, substrate
concentration, pH of buffer, particle size of substrate and incubation temperature on the
hydrolysis of pineapple peel to glucose was studied in a batch process. Within the limits of
experimental conditions, the results show that an optimum temperature of 45 ⁰C, optimum pH
range of 3.8-4.2 and optimum particle size of 100-150 μm were best conditions for the process.
Furthermore, as substrate concentration increases from 10 g/l to 35 g/l, glucose concentration
increases from 0.813 g/l to 2.32 g/l and yield was 8.13% and 6.63% respectively after an
incubation time of two hours. Michaelis-Menten kinetic model was found to adequately
represent the experimental data having a standard deviation of 0.001. The corresponding kinetic
parameters are: maximum rate of reaction, rmax = 0.021 g/l.s and Michaelis-Menten constant,
KM =44.91 g/l at a process condition of 45 ⁰C and pH 4.0.
Description
A DISSERTATION SUBMITTED TO THE
POSTGRADUATE SCHOOL,
AHMADU BELLO UNIVERSITY, ZARIA
NIGERIA
IN PARTIAL FULFILMENT FOR THE AWARD
OF MASTER DEGREE IN
CHEMICAL ENGINEERING
Keywords
KINETIC, STUDY, PINEAPPLE, (Ananas comosus), PEEL CONVERSION, GLUCOSE, Aspergillus niger