IN VITRO FREE RADICAL SCAVENGING AND DNA PROTECTING ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS OF LEAF AND STEM BARK OF Gossypium barbadense
IN VITRO FREE RADICAL SCAVENGING AND DNA PROTECTING ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS OF LEAF AND STEM BARK OF Gossypium barbadense
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Date
2016-11
Authors
ADEWUSI, ADEOLA MARY
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Abstract
Prevention and treatment of various degenerative diseases using traditional medicines is increasingly generating interest, especially in clinical research. This study was aimed to evaluate the invitro free-radical scavenging properties and potential to prevent DNA damage (from oxidative stress) of leaf and stem bark extracts of Gossypium barbadense. Quantitative and Qualitative phytochemical test of different solvent extracts (hexane extract, ethyl acetate extract and aqueous extract) was carried out and result showed that hexane leaf extract had the highest amount of flavonoids (1734.54 ± 6.36 μg equivalent Quercertin/g dry material) and ethyl acetate leaf extract has the highest phenolic content (86.04 ± 0.85 μg Gallic acid/g of dry sample). Scavenging of DPPH radical by all the extracts was evaluated to estimate their antioxidant potential. All the extracts possess free radical scavenging property with hexane leaf extract having the lowest IC50 value of 0.192 ± 0.076 mg/ml; as compared to the standard, ascorbic acid with IC50 value of 0.173 ± 0.098 μg/ml. The IC50 value is in the increasing order of HL > EL > ES > HS > AqL > AqS. Protection of DNA against free radicals-induced oxidative damage (free radicals from Fentons reagent and photolyzed H2O2) by all extracts was studied using Pmax Green Florescence Protein (GFP) plasmid. The result showed that only the aqueous leaf extract had DNA protecting activity. Membrane stabilization test – a measure of anti-inflammatory activity was also carried out. It was observed that all the extracts protected the RBC from hemolysis as compared with a standard, aspirin
Description
A RESEARCH THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA IN PARTIAL FULFILLMENT FOR THE AWARD OF MASTER OF SCIENCE IN BIOCHEMISTRY DEPARTMENT OF BIOCHEMISTRY FACULTY OF SCIENCE AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA
Keywords
VITRO FREE RADICAL SCAVENGING,, DNA PROTECTING ACTIVITIES,, DIFFERENT SOLVENT EXTRACTS,, LEAF,, STEM BARK,, Gossypium barbadense,