DETECTION OF LECTIN GENE IN Piliostigma thonningii AND Albizia lebbeck SEEDS AND THE ANTIMICROBIAL PROPERTIES OF THE PARTIALLY PURIFIED LECTIN

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dc.contributor.authorNWOSU, Oluchi Ekwutosi
dc.date.accessioned2015-11-13T08:55:12Z
dc.date.available2015-11-13T08:55:12Z
dc.date.issued2015-06
dc.descriptionA THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILMENT OF THE REQUIREMENT FOR THE AWARD OF A MASTER OF SCIENCE DEGREE IN BIOCHEMISTRY DEPARTMENT OF BIOCHEMISTRY FACULTY OF SCIENCE AHMADU BELLO UNIVERSITY, ZARIA NIGERIAen_US
dc.description.abstractLectins were extracted from the seeds of Piliostigma thonningii and Albizia lebbeck, using phosphate-buffered saline (PBS) at pH 7. Agglutination reaction using chicken erythrocytes was performed to determine the presence of lectin. Purification of the extract was accomplished by ammonium sulfate precipitation and gel filtration using Sephadex G-75. The purified P. thonningii lectin (PTL) and A. lebbeck lectin (ALL), obtained when treated with denaturing and reducing conditions in the presence of sodium dodecyl sulphate (SDS) and 2-β, mercaptoethanol were electrophoresed using Poly Acrylamide Gel Electrophoresis (SDS-PAGE). The purified protein for A. lebbeck lectin, had a molecular weight of 21kDa as estimated from the standard protein marker while that of P. thonningii lectin, no distinctive band was observed. Both plant seeds crude protein and the partially purified lectin showed no antimicrobial activity against the microorganism (Staphylococcus aureus, Escherichia coli, Salmonella typhi, Bacillus subitilis and a fungi, Candida albicans) tested. The DNA of each plant was isolated using the Zymo Research Plant/Seed Kit, and the optimized Polymerase Chain Reaction used to detect the gene that codes for a galactose specific lectin protein using the primer pair LEC5 (Forward) and LEC6 (Reverse) produced a 195bp product confirming the presence of lectin gene in both plant seeds. This study has successfully confirmed the presence of lectin in both plants using the conventional hemagglutination reaction and Polymerase Chain Reaction, a method that can be applied to a variety of samples for a sensitive and specific detection of lectin in plants.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/7154
dc.language.isoenen_US
dc.subjectDETECTION,en_US
dc.subjectLECTIN GENE,en_US
dc.subjectPiliostigma thonningii,en_US
dc.subjectAlbizia lebbeck,en_US
dc.subjectSEEDS,en_US
dc.subjectANTIMICROBIAL,en_US
dc.subjectPROPERTIES,en_US
dc.subjectPARTIALLY PURIFIED,en_US
dc.subjectLECTIN.en_US
dc.titleDETECTION OF LECTIN GENE IN Piliostigma thonningii AND Albizia lebbeck SEEDS AND THE ANTIMICROBIAL PROPERTIES OF THE PARTIALLY PURIFIED LECTINen_US
dc.typeThesisen_US
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