SCREENING AND IMPROVEMENT OF ASPERGILLUS SPECIES FOR PECTINASE PRODUCTION

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dc.contributor.authorELUDIRE, JIDE OLUFUNMINIYI
dc.date.accessioned2014-02-05T09:39:34Z
dc.date.available2014-02-05T09:39:34Z
dc.date.issued2000-12
dc.descriptionA THESIS SUBMITTED TO THE POSTGRADUATE SCHOOL AHMADU BELLO UNIVERSITY, Z.ARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE DEPARTMENT OF MICROBIOLOGY FACULTY OF SCIENCE AHMADU BELLO UNIVERSITY ZARIA NIGERIA DECEMBER, 2000en_US
dc.description.abstractFour strains of Aspergillus Species isolated from rotten orange, pineapple. cucumber and carrot were screened for pectolytie activities with the use of peetinase detection media; MP - 7 and MP - 5 flooded with pectin precipitant Cetyltrimethvl Ammonium Bromide (CTAB). Isolates from orange and pineapple showed high level of pectolytie activity based on their zones of hydrolysis. These isolates from orange and pineapple with high level of activity were identified to be A. flavus and A. fumigalus respectively and subjected to physical and chemical mutagenesis for improved pectolytie activity. Ultraviolet irradiation and Nitrous acid were used as physical and chemical mutagens respectively (HNO2, UV-10, UV-20 and UV-30). The wild and mutant strains of these organisms were subsequently screened for improved peetinase (pectin lyase and polygalacturonase) production, and calabolilc repression resistance on detection media mentioned above, with and without glucose. Hyperproducers and calabolilc repression resistant mutants were isolated in various cases. Aspergillus fumigants did not yield any diabolical repression resistant mutant for polyunsaturate production, but a hyper-producer was obtained from UV-30. In the production of pectin lyase, a hyper-producer and metabolite repression resistant mutant were obtained from UV - 30. Mutants obtained with nitrous acid did not yield a hyper producer but gave a metabolite repression resistant mutant. A. flavus mutants did not show any hyper- producing ability in the production of polyunsaturate but the nitrous acid and UV-induced mutants were metabolite repression resistant for the enzyme production. For pectin lyase production by this organism, hyperproducers were isolated from nitrons acid and UV-30 mutants, but only UV-30 mutant was resistant to catabolite repressionen_US
dc.identifier.urihttp://hdl.handle.net/123456789/251
dc.language.isoenen_US
dc.subjectSCREENING,en_US
dc.subjectIMPROVEMENT,en_US
dc.subjectASPERGILLUS,en_US
dc.subjectSPECIES,en_US
dc.subjectPECTINASE,en_US
dc.subjectPRODUCTIONen_US
dc.titleSCREENING AND IMPROVEMENT OF ASPERGILLUS SPECIES FOR PECTINASE PRODUCTIONen_US
dc.typeThesisen_US
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