COMPARATIVE EVALUATION OF ANTIBODY PRODUCTION IN BROILERS VACCINATED WITH NEWCASTLE DISEASE VACCINES IN KANO METROPOLIS, NIGERI
COMPARATIVE EVALUATION OF ANTIBODY PRODUCTION IN BROILERS VACCINATED WITH NEWCASTLE DISEASE VACCINES IN KANO METROPOLIS, NIGERI
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Date
2016-08
Authors
SERIKI, Olubukula Olukemi
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Abstract
The incidence of Newcastle disease (ND) in Nigeria is high and is a persistent cause of mortality and (or) morbidity among vaccinated chickens causing huge economic losses. This study was carried out to evaluate the persistence of maternally derived antibody (MDA) against Newcastle Disease Virus (NDV), thermo-stability of the test vaccines, comparative analysis of humoral immune response against four La Sota NDV vaccines as well as the effect of time with respect to vaccination schedule. In study group I; four experimental groups A, B, C and D each which consisted of 10 birds were vaccinated with corresponding vaccines on day 14 (primer) and 28 (booster) and group E of 10 birds was maintained as unvaccinated control. Serum samples collected from five randomly selected birds among the vaccinated groups on day 17, 21, 24, 28 and 35 and from the unvaccinated group E on day 1, 3, 10, 14, 24, 28 and 35 were subjected to haemagglutination inhibition (HI) titre test. Study group II; two experimental groups i and ii consisted of 10 birds each primed on day 14 and boosted on day 35 with vaccines A and B. Serum samples were obtained on day 17, 21, 28 35 and 42 and HI titre measured. The test vaccines were subjected to varying storage temperature and Haemagglutination (HA) titre measured. It was found that MDA against NDV persisted till day 24. The results showed a significant decrease (p<0.05) in HI titre of chickens which were vaccinated at day 14 and 28. Chickens vaccinated at day 14 and boosted at day 35 produced better immune response. Haemaggltuination (HA) titre measured after vaccines were subjected to high temperature was as low as 0. This study has shown that MDA can serve as protection against infective form NDV in chickens for first two weeks of life and none of the test La Sota vaccine was thermo-stable. In conclusion, primary vaccination at day 14 followed by booster dose at day 35 may be followed for better immune response and protection against ND in broilers.
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A DISSERTATION SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA. IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF THE DEGREE OF MASTER OF SCIENCE IN MICROBIOLOGY DEPARTMENT
Keywords
COMPARATIVE EVALUATION,, ANTIBODY PRODUCTION,, BROILERS VACCINATED,, NEWCASTLE DISEASE VACCINES,, KANO METROPOLIS,, NIGERIA