MOLECULAR DETECTION OF WOLBACHIA AND FILARIAL NEMATODE IN ANTHROPOPHILIC MOSQUITOES IN THE GAMBIA
MOLECULAR DETECTION OF WOLBACHIA AND FILARIAL NEMATODE IN ANTHROPOPHILIC MOSQUITOES IN THE GAMBIA
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Date
2018-04
Authors
SANNEH, SANKUNG
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Abstract
This study was embarked upon to provide a baseline information on the incidence of Wolbachia in anthropophilic mosquitoes as well as to provide information on active transmission of lymphatic filariasis in The Gambia using polymerase chain reaction. The study was conducted in 48 settlement ( 8 from each division). A total of 2003 mosquitoes pooled into 120 clusters from the 48 settlements in the country were classified by microscopic technique into genera. The DNA of the mosquitoes by cluster were examined using polymerase chain reaction and gel electrophoresis for the presence of Wolbachia and filarial nematode using 16SrDNA for wolbachia and SspI for Wuchereria bancrofti and HhaI for Brugia malayi. The positive samples for Wolbachia were further differentiated into supergroup of wolbachia using the primer wsp136F/wsp691R for supergroup A and primer wsp81F/wsp522R for supergroup B. The level of association between wolbachia and the genera of anthropophilic mosquitoes in the Gambia was also determined. Microscopic results shows 64.9% Anopheles, 32.0% Culex, 3.0% Aedes. There were no Mansonia mosquito genera detected. Using 16srDNA primers, an incidence of 34.17% of Wolbachia in the mosquito were obtained which varied among the three genera, highest being among Anopheles. Only Wolbachia supergroup B was identified in Culex and Ades. The level of association was found to be significant (P≤ 0.05) only in Culex anthropophilic mosquitoes. For the 120 cluster of mosquitoes screened for the presence of Wuchereria bancrofti and Brugia malayi, non of the clusters were positive.
Description
A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES.
AHMADU BELLO UNIVERSITY , ZARIA
IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD
OF A
MASTER OF SCIENCE (BIOTECHNOLOGY)
DEPARTMENT OF BIOCHEMISTRY
FACULTY OF LIFE SCIENCE
AHMADU BELLO UNIVERSITY , ZARIA
NIGERIA
Keywords
MOLECULAR DETECTION,, WOLBACHIA,, FILARIAL NEMATODE,, ANTHROPOPHILIC MOSQUITOES,, GAMBIA