EFFECT OF CO-ADMINISTRATION OF METFORMIN AND CEFIXIME ON SOME BIOMARKERS IN DIABETIC RATS
EFFECT OF CO-ADMINISTRATION OF METFORMIN AND CEFIXIME ON SOME BIOMARKERS IN DIABETIC RATS
No Thumbnail Available
Date
2011-05
Authors
OLURISHE, COMFORT OMOIGEMETE
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The incidence of infections in Type 2 Diabetes Mellitus (T2DM) continues to be on the increase, necessitating the combined use of antibiotics and hypoglycaemic agents. Metformin, a biguanide is the first drug of choice in the management of T2DM and is most frequently employed with antibiotics when infections exist as co morbid state. This study investigated the effect of Metformin (200 mg/kg) and Cefixime (400 mg/kg) in alloxan (140 mg/kg) induced diabetic rats. Metformin was administered orally for 28 days alone and 14 days co-administration with Cefixime and the effect on some metabolic biomarkers were recorded. The effect of combination of Metformin 4μg/ml, 2μg/ml and 1μg/ml on in vitro antimicrobial activities of Cefixime 5μg/ml against K. species, P. mirabilis, H. influenza and E.coli was also investigated. There was a statistically significant reduction (p<0.05) in Fasting Blood Glucose (FBG) on Day 28 and Random Blood Glucose (RBG) (p<0.01) on day 21 of diabetic rats in Metformin/Cefixime group compared to Metformin alone group. There were no statistically significant changes in the haematological (WBC, RBC and PCV) and biochemical (urea, creatinine and electrolytes) parameters studied. There was no significant difference in body weight and relative weights of selected organs in the drug treated groups and the control. Histopathological findings revealed various degrees of pathological changes in the liver and kidney of the diabetic rats in Metformin and Metformin/ Cefixime group. The pancreas showed areas of pancreatic acini with less cellular endocrine portion in Metformin alone group. Metformin did not significantly alter the in vitro antimicrobial activities of Cefixime against the selected Microrganism used in this study. The study showed that Cefixime may slightly increase the hypoglycaemic activity of Metformin when co-administered. This effect may not appear to be of much clinical significance when both drugs are administered at therapeutic doses, but will call for caution when used for a longer period or in other situations predisposing patients to possible accumulation of Metformin.
Description
A THESIS SUBMITTED TO THE POSTGRADUATE SCHOOL AHMADU BELLO UNIVERSITY, IN PARTIAL FUFILLMENT OF THE REQUIRMENTS FOR THE AWARD OF MASTERS IN PHARMACOLOGY
DEPARTMENT OF PHARMACOLOGY AND THERAPEUTICS
FACULTY OF MEDICINE ,
AHMADU BELLO UNIVERSITY,
ZARIA, NIGERIA